摘要
以巯基丙酸(MPA)为稳定剂,水相合成了高荧光CdTe量子点.向量子点溶液中加入Mn2+,由于Mn2+与量子点之间发生电子转移而使其荧光猝灭,加入抗坏血酸后量子点荧光又得以恢复,且荧光恢复程度与抗坏血酸的浓度线性相关,从而建立了基于量子点的荧光"开关"探针检测抗坏血酸的新方法.在优化的实验条件下,当CdTe量子点的浓度为1.67μmol/L(量子点的尺寸为1.91 nm),Mn2+浓度为0.25 mmol/L时,抗坏血酸的线性检测范围为0.25～16μmol/L,检出限为36 nmol/L,相对标准偏差为2.6%(10μmol/L,n=11).该探针可用于维生素C片剂和人血浆中抗坏血酸的快速、灵敏和选择性检测.
CdTe quantum dots of highly fluorescent was prepared in aqueous solution with mercaptopropanoic acid（MPA） as stabilizer.The fluorescence of the CdTe QDs was significantly quenched with blue shift by the addition of Mn2＋ due to the change of surface structure and chemical properties of the QDs.The fluorescence recovery was afterwards achieved in the presence of a small amount of ascorbic acid,which provided a novel QD-based turn-on fluorescent probe for the detection of ascorbic acid.When using QDs of 1.91 nm in size and a concentration of 1.67 μmol/L in the presence of 0.25 mmol/L Mn2＋,the recovered fluorescence was linearly related with the ascorbic acid concentration within 0.25—16 μmol/L,with a detection limit of 36 nmol/L.The relative standard deviation was derived to be 2.6% at 10 μmol/L of ascorbic acid（n=11）.The probe has been applied for the detection of ascorbic acid in Vc tablets and human whole blood samples.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2011年第3期688-693,共6页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20805004)
国家杰出青年科学基金(批准号:20725517)
中央高校基本科研业务费(批准号:N090105001
N090605001
N090405004
N100305003
N100305004
N100605002)资助
