摘要
目的:检测肺炎支原体(mycoplasma pneumoniae,MP)感染患儿细胞免疫和体液免疫功能,研究其变化规律,探讨疾病发生机制。方法:收集支原体肺炎患儿急性期全血和血清以及健康儿童对照标本,采用流式细胞仪检测淋巴细胞表面标记CD3+、CD4+、CD8+、NK、CD19+、CD3+HLA-DR+、CD3+CD25+、CD8+HLA-DR+、CD8+CD38+和调节性T细胞CD4+CD25+表达比例。采用IMMAGE特定蛋白分析仪检测血清标本IgG、IgA、IgM、C3和C4浓度。结果:与正常对照组比较,支原体肺炎患儿组急性期外周血CD3+、CD8+CD38+和CD4+CD25+细胞比例明显下降,B淋巴细胞(CD19+细胞)比例则显著升高,血清中IgG和IgA含量明显下降。结论:支原体肺炎患儿急性期处于免疫失衡状态,T淋巴细胞增殖受到抑制,B淋巴细胞刺激增殖,体液免疫功能仍存在一定失调。
Objective：The cellular immunity and humoral immunity function of children infected with mycoplasma pneumoniae（MP） were detected to investigate the changes,index and the mechanism of this disease.Methods：Peripheral blood and serum samples of healthy children and children infected with MP in acute stage were collected,CD3^＋,CD4^＋,CD8^＋,NK,CD19^＋,CD3^＋HLA-DR^＋,CD3^＋CD25^＋,CD8^＋HLA-DR^＋,CD8^＋CD38^＋ and CD4^＋CD25^＋ were detected by Flow Cytometry.The serum concentration of IgG,IgA,IgM,C3 and C4 were detection by IMMAGE.Results：The proportion of CD3^＋,CD8^＋CD38^＋,CD4^＋CD25^＋ and the concentration of both IgG and IgA in MP group were much lower than the healthy group,while the proportion of CD19^＋ was significant higher than the healthy group.Conclusion：The immune system of mycoplasmal pneumonia children is in imbalance status,T lymphocytes are greatly suppressed,especially CD8^＋ T cells.B cells are activated.The humoral immunity function is still in imbalance with the decrease of IgG and IgA.
出处
《中国卫生检验杂志》
CAS
2011年第1期126-127,共2页
Chinese Journal of Health Laboratory Technology
关键词
肺炎支原体
流式细胞仪
细胞免疫
体液免疫
Mycoplasma Pneumoniae
Flow Cytometry
Cellular Immunity
Humoral Immunity
