摘要
以富营养化官厅水库(北京)和天然Rupa湖(尼泊尔)沉积物为对象,使用16Sr DNA克隆文库的分子生态学方法,鉴定并比较了其中的细菌群落结构。分析表明:二者pH及各形态磷含量参数差异显著。在细菌组成上,二者的主要种群均为变形杆菌门,且均以β-变形杆菌纲为主(比例高于50%),但Rupa湖沉积物中以α-变形杆菌次多,而官厅水库沉积物以γ-变形杆菌次多,2个样品中δ-变形杆菌的含量均相对较少。其他种群组成上,官厅水库沉积物中绿弯菌纲(9.21%)含量高于Rupa湖(1%),并特有芽单胞菌纲(12.2%)、放线菌纲(1.3%)和芽孢杆菌纲(0.8%)。而Rupa湖沉积物中特有的种群为螺旋菌纲(2%)、绿菌纲(1%)、梭菌纲(1%)和疣微菌纲(0.3%)。官厅水库和Rupa湖沉积物均含有少量的酸杆菌纲细菌。
Bacterial communities of sediments from Guanting Reservoir（Beijing） and Rupa Lake（Nepal） were studied using method of 16S rDNA clone library.In the sediments,pH and concentration of total phosphorus and phosphorus fractions were obviously different.Bacterial communities of the two samples were both dominant by phylum of Proteobacterium,in which the percentage of β-proteobacterium was highest（50%）.The second dominant bacteria cluster of sediment from Rupa Lake is α-Proteobacterium,other than γ-Proteobacterium in that from Guanting Reservoir.δ-Proteobacterium in both samples was much less.In sediment from Guanting Reservoir,percentage of Chloroflexi（9.21%） was much higher than that of Rupa Lake（1%）,and Gemmatimonadetes（12.2%）,Acidobacteria（1.3%） and Bacilli（0.8%） were peculiar.Sediment of Rupa Lake is peculiar of Spirochaetes（2%）,Chlorobi（1%）,Clostridia（1%） and Verrucomicrobiae（0.3%）.There were less Actinobacteridae in sediment of Guanting Reservoir and Rupa Lake.
出处
《中国农业大学学报》
CAS
CSCD
北大核心
2011年第1期49-53,共5页
Journal of China Agricultural University
基金
国家自然科学基金项目(31070105)
江苏省太湖水污染治理科技专项项目(BK2007741)
国家青年基金项目(31000065)
关键词
水资源
细菌群落
沉积物
富营养化
water resource
bacterial community
sediment
eutrophication
