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结核分枝杆菌抗原Rv1258c、Rv1410c及Rv3425的HLA限制性CTL表位预测 被引量：3

Prediction of HLA restricted CTL epitopes in Mycobacterium tuberculosis antigens Rv1258c,Rv1410c and Rv3425
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摘要 目的:预测结核分枝杆菌(MTB)相关抗原Rv1258c、Rv1410c及Rv3425的HLA限制性细胞毒性T细胞(CTL)表位。方法:通过NCBI数据库获取各蛋白的氨基酸序列,利用BLAST分析其与人类蛋白质的同源性,利用SYFPEITHI、BIMAS和NetCTL数据库预测分析MTB相关抗原的HLA-A2与HLA-A3限制性CTL表位。结果:初步筛选出3个候选抗原的HLA-A2和HLA-A3限制性CTL优势表位,分别为Rv1258c343～351、380～388、290～298、52～60;Rv1410c510～518、470～478、452～460、420～428;Rv3425118～126、43～51、29～37。结论:预测出MTB3个相关抗原的HLA-A2与HLA-A3限制性CTL表位,为抗结核多表位疫苗的研究奠定了基础。 Aim：To predict the HLA restricted cytotoxic T lymphocyte（CTL）epitopes in Mycobacterium tuberculosis antigens Rv1258c,Rv1410c and Rv3425.Methods：The amino acid sequences of the antigens were obtained from NCBI database.Their homologies to human proteins were analyzed by BLAST.HLA-A2-and HLA-A3-restricted CTL epitopes were predicted by SYFPEITHI,BIMAS and NetCTL.Results：The predominant HLA-A2-and HLA-A3-restricted CTL epitopes predicted from the three candidate antigens were Rv1258c 343~351,380~388,290~298,52~60;Rv1410c 510~518,470~478,452~460,420~428;and Rv3425 118~126,43~51,29~37.Conclusion：Eleven HLA-A2-and HLA-A3-restricted CTL epitopes have been predicted from Mycobacterium tuberculosis antigens Rv1258c/Rv3425/Rv1410c,which could be used in research of antituberculosis multi-epitope vaccine.
作者 朱宇皇 高艳锋 吕虹 祁元明
机构地区 郑州大学生物工程系
出处 《郑州大学学报（医学版）》 CAS 北大核心 2011年第1期13-15,共3页 Journal of Zhengzhou University(Medical Sciences)
基金 国家自然科学基金资助项目30872381 河南省杰出人才基金资助项目084200510014
关键词 结核分枝杆菌 Rv1258c Rv3425 Rv1410c 表位预测 Mycobacterium tuberculosis Rv1258c Rv3425 Rv1410c epitope prediction
分类号 R52 [医药卫生—内科学]
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参考文献13

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郑州大学学报（医学版）
2011年 第1期

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