摘要
采用穗培养技术在7个氮素(NH4NO3为氮源)浓度水平(N1:0mg/L;N2:412.5 mg/L;N3:825.0mg/L;N4:1237.5mg/L;N5:1650.0mg/L;N6:3300.0mg/L;N7:4950.0mg/L)下研究了氮素对2个大麦品种籽粒中酚酸和蛋白质含量的影响。结果表明,在高氮水平(N6和N7)下,大麦籽粒发育受到明显胁迫,粒重显著降低。氮素浓度、品种及氮素浓度与品种的互作等因素均显著影响大麦籽粒中总羟基苯甲酸衍生物含量(THBA)、总羟基肉桂酸衍生物含量(THCA)、总酚酸含量(TPA)及THBA/THCA比值,而且4个指标与氮素浓度显著正相关。氮素浓度显著影响籽粒中粗蛋白含量(CPC)、盐溶蛋白含量(SSPC)和谷蛋白含量(GC),但对醇溶蛋白含量(HC)的影响不显著;品种、氮素浓度与品种的互作对SSPC和GC的影响显著,但对CPC和HC的影响不显著。相关分析表明氮素浓度与CPC和SSPC显著正相关,而与HC和GC的相关性不显著。本研究还发现,随着氮素浓度水平的提高,大麦籽粒中酚酸含量(包括THBA、THCA和TPA)与CPC和SSPC有协同增加的趋势。
Nitrogen is one of the necessary nutrient elements for crop growth.The primary and secondary metabolisms of plant are regulated by nitrogen nutrient levels.As important secondary metabolites,phenolic acids play significant roles in plant resistances,and potentially contribute to barley qualities.However,little is known concerning effects of nitrogen on barley（Hordeum vulgare L.） phenolic acids.In the present study,two barley cultivars were used,and seven nitrogen concentration treatments（0,412.5,825.0,1237.5,1650,3300,and 4950mg/L,respectively） under in vitro spike culture were conducted to investigate the effects of nitrogen on phenolic acid and protein contents in barley grain.Results showed that the development of barley grain was inhibited under high nitrogen levels（3300 ～ 4950mg/L）,and grain weight decreasing significantly was as the response.Total hydroxybenzoic acid content（THBA）,total hydroxycinnamic acid content（THCA）,total phenolic acid content（TPA） and THBA/THCA ratio were all significantly affected by nitrogen concentration,cultivar and the interaction between nitrogen concentration and cultivar.Four indexes above were all significantly positively correlated with nitrogen concentration.Except for hordein content（HC）,the protein indexes of crude protein content（CPC）,salt-soluble protein content（SSPC）,and glutelin content（GC） were all significantly affected by nitrogen concentration,moreover,SSPC and GC were significantly affected by cultivar and the interaction between nitrogen concentration and cultivar.Excluding HC and GC in the four indexes,CPC and SSPC were significantly positive-correlated with nitrogen concentration.The results also indicated that phenolic acid contents （including THBA,THCA,and TPA） in barley grain increased cooperated with CPC and SSPC when the nitrogen nutrient level went up.
出处
《核农学报》
CAS
CSCD
北大核心
2011年第1期162-168,共7页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金项目(30760112)
现代农业产业技术体系建设专项资金
关键词
氮素
酚酸
蛋白质
大麦
nitrogen
phenolic acids
protein
barley（Hordeum vulgare L.）
