摘要
目的: 检测以恶性疟原虫裂殖子表面蛋白1 的17 区片段基因为基础的复合核酸疫苗VR1012/TPA/HG-MSP1-17(分泌性)和VR1012/HG-MSP1-17(非分泌性)诱导小鼠的体液免疫反应。方法: 分别用分泌性与非分泌性核酸疫苗肌注免疫小鼠。用间接ELISA法测定小鼠血清的特异性抗体。结果: 每只小鼠经3 次100 μg/100μl非分泌性核酸疫苗免疫后, BALB/c小鼠和C57BL/6 小鼠均产生明显的抗HG和抗真菌表达的MSP1 17 区蛋白(YMSP119 )抗体。但总体抗体水平不高。BALB/c小鼠经3 次200 μg/100 μl非分泌性核酸疫苗免疫后, 产生较高的抗HG抗体, 但抗MSP1-17 的抗体无明显变化。经3 次200 μg/100 μl分泌性核酸疫苗免疫后, 只产生较低的抗HG抗体。结论:
AIM: To analyse the humoral immune response in mice to nucleic acid vaccines ( VR1012/HG MSP1 17 for intracellular expression or VR1012/TPA/HG MSP1 17 for secretion) containing Plasmodium falciparum merozoite surface protein 1 (MSP1) 17 block gene and gene fragment of several T cell epitopes from MSA1, MSA2, RESA, IL 1 and TT. METHODS: BALB/c or C 57 BL/6 mice received three times intramuscular immunization with 200 μg/100 μl or 100 μg/100 μl of VR1012/HG MSP1 17 or VR1012/TPA/HG MSP1 17 per mouse each time. Anti HG or anti MSP1 17 antibodies were monitored by indirect ELISA. RESULTS: BALB/c and C 57 BL/6 mice immunized with 100 μg/100 μl of VR1012/HG MSP1 17 per mouse raised significantly anti HG and anti MSP1 17 antibodies, but the levels of antibodies were not high. BALB/c mice immunized with 200 μg/100 μl of VR1012/HG MSP1 17 per mouse raised higher anti HG antibodies but not anti MSP1 17 antibodies. BALB/c mice immunized with 200 μg/100 μl of VR1012/TPA/HG MSP1 17 per mouse raised low level of anti HG antibodies only. CONCLUSION: VR1012/HG MSP1 17 is more immunogenic than VR1012/TPA/HG MSP1 17.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1999年第5期302-304,共3页
Chinese Journal of Parasitology and Parasitic Diseases
基金
联合国开发计划署/世界银行/世界卫生组织热带病研究和培训特别规划署资助项目!(ID No.950385)
关键词
恶性疟原虫
核酸疫苗
MSP1
分泌性
Plasmodium falciparum, nucleic acid vaccine, merozoite surface protein 1
