摘要
目的:建立棘阿米巴角膜炎的动物模型。方法:在6 只新西兰家兔角膜基质层内注射地塞米松3 d 后,再注入棘阿米巴原虫悬液。结果:6 只家兔均发生角膜炎。经家兔角膜刮片用10% 氢氧化钾封片镜检、角膜组织原虫培养和病理切片染色检查证实,成功地建立了家兔棘阿米巴角膜炎动物模型。结论:通过角膜基质内注射建立的家兔棘阿米巴角膜炎动物模型。
AIM: To establish an animal model of Acanthamoeba keratitis. METHODS:Six New Zealand white rabbits were each injected intrastromally with Acanthamoeba suspension 3 days after subconjunctival injection with dexamethasone. RESULTS:All of the 6 rabbits developed keratitis. Acanthamoeba protozoa were identified by the methods of corneal scraping with 10% potassium hydroxide wet mount examined under microscope,corneal protozoa culture and pathological section examination. CONCLUSION:A rabbit model of Acanthamoeba keratitis was established.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1999年第5期308-310,共3页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
棘阿米巴角膜炎
基质内注射
动物模型
Acanthamoeba keratitis, animal model, intrastromal injection
