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甘油脱水酶的研究进展

Recent Progress in Research of Glycerol Dehydratase
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摘要 甘油脱水酶是微生物发酵法生产3-羟基丙醛和1,3-丙二醇过程中的关键限速酶.文中对甘油脱水酶的结构与功能、作用机制、基因工程研究等情况进行综述,探讨了甘油脱水酶的研究进展并提出一些建议. Glycerol dehydratase(GDHt) is a key rate-limiting enzyme in the process of producing 3-hydroxypropionaldehyde(3-HPA) and 1,3-propanediol(1,3-PD) by microbial fermentation.In this paper,the research in the structure and functions,catalytic mechanism and genetic engineering of GDHt were reviewed.The research progresses of GDHt were also discussed and some suggestions were given in the end.
出处 《华侨大学学报(自然科学版)》 CAS 北大核心 2011年第2期125-129,共5页 Journal of Huaqiao University(Natural Science)
基金 国家高技术研究发展(863)计划项目(2006AA020103) 国家自然科学基金资助项目(20446004 20676048)
关键词 甘油脱水酶 生物转化法 结构功能 作用机制 基因工程 glycerol dehydratase biotransformation structure and functions catalytic mechanism genetic engineering
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  • 1BROWN K L,MARQUES H M.Molecular modeling of the mechanochemical triggering mechanism for catalysis of carbon-cobalt bond homolysis in coenzyme B12[J].Inorg Biochem,2001,83(2/3)..121-132.
  • 2SAINT-AMANS S,GIRBAL L,ANDRADE J,et al.Regulation of carbon and electron flow in Clostridium butyricum VPI 3266 grown on glucose-glycerol mixtures[J].J Bacteriol,2001,183(5):1748-1754.
  • 3RAYNAUD C,SARCABAL P,MEYNIAL-SALLES I,et al.Molecular characterization of the 1,3-propanediol (1,3-PD) operon of Clostridium butyricum[J].PNAS,2003,100(9):5010-5015.
  • 4LIAO D I,DOTSON G,TURNER J I,et al.Crystal structure of substrate free form of glycerol dehydratase[J].Inorg Biochem,2003,93(1/2):84-91.
  • 5TORAYA T.Radical catalysis of B12 enzymes:Structure,mechanism,inactivation,and reactivation of diol and glycerol dehydratase[J].Cell Mol Life Sci,1999,57(1):106-127.
  • 6YAMANISHI M,YUNOKI M,TOBIMATSU T,et al.The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol[J].Eur J Biochem,2002,269 (18):4484-4494.
  • 7DOLKER N,MASERAS F,SIEGBAHN P E M.Stabilization of the adenosyl radical in coenzyme B12:A theoretical study[J].Chem Physi Let,2004,386(1/2/3):174-178.
  • 8洪燕,李雯君,张燎原,夏启容,方柏山.甘油脱水酶和二醇脱水酶β亚基生物信息学分析[J].生物技术,2006,16(4):1-4. 被引量:1
  • 9洪燕,张燎原,李雯君,夏启容,方柏山.依赖辅酶B12的甘油脱水酶生物信息学分析[J].生物信息学,2007,5(3):109-112. 被引量:1
  • 10陈永胜,刘长江,李长彪,翟景波.重组甘油脱水酶的活性鉴定[J].内蒙古民族大学学报(自然科学版),2005,20(2):172-175. 被引量:1

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