摘要
成年雄性Wistar大鼠41只。分为实验性胃溃疡组和对照组,后者又分为盐水对照组和空白对照组。于胃溃疡手术后1、4、10、23d分批取胰尾.以免疫细胞化学PAP法对胃溃疡自愈期间胰岛β-EP免疫反应细胞进行研究。结果:溃疡组β-EP细胞面数密度在术后4、10d均增加,4d达最高峰.与对照相比为P<0.01,23d基本恢复正常。邻片单染显示:β-EP阳性细胞分别与部分Glu、Ins 阳性细胞重叠。本研究结果提示:胰岛β-EP细胞参与了大鼠实验性胃溃疡时期的内分泌调制。
Adult male Waister rats were randomly divided into the experimental gastric group and the control groups.The latter included the sailine control group and the normal group.The pancreatic tail were removed,after the animals were sacrificed at different time intervals(1-23d).Immunohistochemical method were used for showing β-EP immunoreactive cells during self-healing process of the experimential gastric ulcer.The result:The numerical den sitiy on area of β-EP cells in gastric ulcer group were higher than control groups on the 4th and 10th days after opera-tion(P<0.01).It was found that they were overlapped with part of Gluand Ins cells by single stained in neighbour section.The present work further proved that the β-EP cells of pancreatic islets were participated in the regulation bal ance of endocrine during self-healing process of the experimential gastric ulcer.
关键词
实验性胃溃疡
Β-内啡肽
免疫细胞化学
大鼠
胰岛
experimental gastric ulcer β-emndorphin imrnunocyto chemistry rat pancreatic islet
