摘要
为研究氟致非骨相组织的损伤作用,选用20只20日龄的昆明小鼠,随机分为4组,每组5只,分别饲喂含0 mg/L、50 mg/L1、00 mg/L和150 mg/L氟化钠的饮水120 d,并通过石蜡组织切片观察氟对肾脏的损伤作用。研究结果表明:50 mg/L氟中毒组小鼠肾脏肾小管排列结构无异常变化,肾小球细胞出现空泡样病变。100 mg/L氟中毒组小鼠肾脏肾小管排列结构没有明显的异常,肾小球内皮细胞和肾小管上皮细胞出现空泡样变,并出现微核现象和凋亡样细胞,且近曲小管上皮细胞有脱落现象。150 mg/L氟中毒组小鼠肾脏肾小管排列结构轻微紊乱,肾小球内皮细胞和肾小管上皮细胞发生变性、坏死,肾小管内脱落的上皮细胞增多。
In order to analyse toxic effect of fluoride on non - skeletal tissue, 20 Kunming mice with age of 20 days old were selected, and randomly divided into 4 groups with each group 5 mice. The animals were feed respectively with water containing 0 mg/L, 50 mg/L, 100 mg/L and 150 mg/L sodium fluoride. The lesions of renal tissue induced by fluoride were studied by paraffin -embedded tissue specimens. The results showed that the renal tubule has normal arrangement, but vacuolated changes were found within glomerular endothelium in 50 mg/L fluoride -treated group; that the kidney tubule has normal situation as well as vacuolated lesions within glomerular endothelium and tubular epithelium, micronuclei and apoptosis -like cells accompanied by shedding off of tubular epithelium in 100 mg/L fluoride -treated group; that renal tubule has slight dearrangement, degeneration and necrosis of glomerular endothelium and tubular epithelium was observed, and the amount of tubu- lar epithelium shed off from tubule are higher in 150 mg/L fluoride - treated group.
出处
《山东农业大学学报（自然科学版）》
CSCD
北大核心
2011年第1期75-78,共4页
Journal of Shandong Agricultural University：Natural Science Edition
基金
云南高端人才引进计划(2009CI125)
云南省教育厅科学研究基金(09Y0289)
关键词
氟
昆明小鼠
肾
组织切片
形态结构
Fluoride
Kunming mouse
kidney
tissue slice
structure
