摘要
为了将生物超弱光子技术应用于萌发过程中小麦种子的抗旱性评价,采用聚乙二醇处理萌发的小麦种子,跟踪测量了正常和渗透胁迫下小麦种子萌发过程中的超弱光子辐射,得到了小麦种子萌发过程中超弱光子辐射的自发发光和延迟发光信号.通过建立延迟发光动力学方程和数学拟合获得了种子萌发过程中延迟发光初始光强I(0)、衰减参量β、相干时间τ和延迟发光积分强度I(T),根据延迟发光积分强度和自发发光的生物学意义构建了用于描述细胞状态及其有序性的状态参量和序参量.实验结果发现:小麦种子萌发过程中,自发发光和延迟发光积分强度呈现阶跃式增长,细胞系统的状态参量和序参量也呈现阶跃式的增长,渗透胁迫抑制了这种增长,提示基于光子辐射的细胞系统的状态参量和序参量有可能作为种子抗旱性评价的物理指标.
In order to evaluate drought resistance of wheat seed during germination using bio-photonic technology,treatment to germinating wheat seeds with polyethylene glycol,spontaneous luminescence and delayed luminescence of wheat seed in ultra-weak photon emission during germination under osmotic stress were measured.Through establishment of dynamic equation of delayed luminescence and mathematical fitting,the initial intensity I（0）,attenuation parameters β,coherence time τ and integral intensity I（T） of delayed luminescence during germination were obtained.And the state parameters and the order parameter of cell system were constructed according to the significance of delayed luminescence integral intensity and spontaneous luminescence.The expermental results shows that the increases of spontaneous luminescence and integrated intensity of delayed luminescence in ultra-weak photon emission of wheat seed are the stage during germination,the increases of the state parameter and order parameter of cell are the stage,and osmotic stress inhibits these increases.It is suggested that the state parameter and order parameter of cell based on ultra-weak photon emission may be used as physical indicators to evaluate drought resistance of wheat seed during germination.
出处
《光子学报》
EI
CAS
CSCD
北大核心
2011年第2期282-288,共7页
Acta Photonica Sinica
基金
国家自然科学基金(No.50977079)
陕西省教育厅专项科研计划(No.09JK667)资助
关键词
超弱光子辐射
渗透胁迫
小麦萌发
序参量
Ultra-weak photon emission
Osmotic stress
Wheat germination
Order parameter
