摘要
目的:研究金银花药材总脱氧核糖核酸(DNA)的提取方法。方法:对经典的十六烷基三甲基溴化铵(CTAB)法进行了改良,采用改良的CTAB法提取金银花药材的总DNA,通过紫外吸收、琼脂糖凝胶电泳检测其纯度和浓度。结果:用改良的CTAB法提取的总DNA的OD260/OD280比值在1.76-1.85之间,干药材提取率为89.8-325.4μg/g。结论:用改良CTAB法提取的基因组DNA纯度高,符合分子生物学的要求。
Objective：To research the method of extracting DNA from Flos Lonicerae Japonicae.Methods：The modified classical CTAB method.The quality and purity of the extracted DNA were detected by ultraviolet absorption and agarose gel electrophoresis.Results：The OD260/OD280 ratios of genomic DNA extracted with the modified CTAB method ranged from 1.76to 1.85,the yields of DNA sample were in the rang of 89.8-325.4 μg/g.Conclusion：The DNA samples extracted with the modified CTAB method are of high quality and suitable for molecule biological research.
出处
《中医学报》
CAS
2011年第2期188-189,共2页
Acta Chinese Medicine
基金
河南省郑州市科技创新团队基金(编号:2009)
