摘要
在对玉米低聚肽的蛋白质含量、氨基酸组成和分子质量分布进行分析的基础上,从DPPH自由基、羟自由基、超氧阴离子自由基清除能力以及还原能力4个方面对玉米低聚肽的体外抗氧化活性进行考察。组成分析显示:总蛋白质含量为89.28%,酸溶蛋白质占总蛋白质含量的94.31%;氨基酸组成独特,富含抗氧化性氨基酸,其中亮氨酸、脯氨酸、丙氨酸含量较高;相对分子质量小于1000的组分高达93.05%,主要分布在132～576范围内。体外抗氧化实验结果显示:玉米低聚肽对DPPH自由基、羟自由基和超氧阴离子自由基的半抑制浓度(IC50值)分别为:1.9、5.4mg/mL和14.9mg/mL,质量浓度为5.0mg/mL时的还原能力与0.02mg/mL的抗坏血酸相当,并且抗氧化能力与其质量浓度呈现剂量关系。
On the basis of protein content,amino acid composition and molecular weight distribution of corn oligopeptides（COP）,the antioxidant activity of COP in vitro was evaluated by determining the abilities to scavenge DPPH,hydroxyl and superoxide anion free radicals as well as reducing power.Results indicated that the content of total protein was 89.28%,of which acid soluble proteins accounted for 94.31%;amino acid composition was unique and COP was rich in amino acids such as leucine,proline and alanine with strong antioxidant activity.The major molecular weight of COP was less than 1000（93.05%）,and small peptides with a molecular weight range of 132-576 were dominant.The median inhibitory concentrations（IC50 values） of COP for scavenging DPPH,hydroxyl and superoxide anion free radicals on the basis of scavenging rate were 1.9,5.4 mg/mL and 14.9 mg/mL,respectively.The reducing power of COP at a concentration of 5.0 mg/mL was similar to that of ascorbic acid at 0.02 mg/mL.The antioxidant activity of COP exhibited a dose-dependent relationship.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第5期22-26,共5页
Food Science
基金
国家"863"计划项目(2007AA10Z327)
关键词
玉米
低聚肽
氨基酸组成
分子质量分布
抗氧化活性
corn
oligopeptide
molecular weight distribution
amino acid composition
antioxidant activity
