摘要
建立了测定啤酒和鲱鱼精DNA中的碱基和核苷的阳离子交换色谱法。采用强阳离子交换柱,以水(A)和稀H2SO4(B,pH 2)为流动相,梯度洗脱:0～15 min,0～50%B。6种核苷和碱基(次黄嘌呤、鸟苷、胞苷、胞嘧啶、鸟嘌呤和腺嘌呤)在18 min内实现基线分离,UV检测波长为254 nm。实验表明,6种核苷和碱基的工作曲线的线性范围为0.1～100 mg/L;检出限为0.009～0.068 mg/L(S/N=3),峰面积和保留时间的相对标准偏差(RSD)分别为1.3%～2.2%和1.6%～3.1%。将本方法用于啤酒和鲱鱼精DNA中的碱基和核苷的分析,平均回收率为89.5%～102.9%。
A cation exchange chromatographic method was developed for the determination of six nucleosides and bases in beer and herring sperm DNA.The separation was carried out on a strong cation exchange column with water（A） and pH=2 H2SO4 solution（B） as mobile phase.The gradient elution program was： 0-15min,0-50% B.Baseline separation of six nucleosides and bases（hypoxanthine,guanosine,cytidine,cytosine,guanine and adenine） were obtained within 18 min.The UV detection wavelength was 254 nm.As a result,good linear relationships were obtained in the range of 0.1-100 mg/L;the detection limits of six analytes were in the range of 0.009-0.068 mg/L（S/N=3）.The relative standard deviations（RSD） of the peak areas and the retention times were in the range of 1.3%-2.2% and 1.6%-3.1%,respectively.This method has been successfully applied to the simultaneous determination of six nucleosides and bases in beer and herring sperm DNA with the average recoveries in the range of 89.5%-102.9%.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2011年第3期405-408,共4页
Chinese Journal of Analytical Chemistry
关键词
阳离子交换色谱法
核苷
碱基
啤酒
鲱鱼精脱氧核糖核酸
Cation-exchange chromatography
Nucleosides
Bases
Beer
Herring sperm deoxyribonucleic acid
