摘要
为了明确宁夏干旱带压砂甜瓜白粉病病原菌,从病原菌分生孢子中提取DNA,PCR扩增ITS rDNA和28S rDNA D1/D2区段,测序后进行BLAST比对。结果表明,病原菌的ITS rDNA和28S rDNA D1/D2序列与菜豆叉丝单囊壳白粉菌Podosphaera phaseoli、凤仙花叉丝单囊壳白粉菌P.bal-sam inae、菊科叉丝单囊壳白粉菌P.fusca、苍耳单囊壳白粉菌P.xanthii、瓜类单囊壳白粉菌P.fuligi-nea等叉丝单囊壳属Podosphaera的多个种的ITS rDNA和28S rDNA D1/D2序列之间相似度均大于99%,鉴定甜瓜白粉病病原菌为叉丝单囊壳属Podosphaera。
In order to investigate the pathogen of melon powdery mildew in lands overlaid with sands in Ningxia droughty area,ITS rDNA and 28S rDNA D1/D2 of pathogen amplified by PCR were sequenced and analyzed by BLAST in GenBank after DNA had been extracted from conidia of pathogen.The results showed that ITS rDNA and 28S rDNA D1/D2 sequence of pathogen had more than 99% of similarity to the species of Podosphaera phaseoli,P.balsaminae,P.fusca,P.xanthii,P.fuliginea,suggesting the pathogen of melon powdery mildew belonged to Podosphaera.
出处
《植物保护学报》
CAS
CSCD
北大核心
2011年第1期47-51,共5页
Journal of Plant Protection
基金
宁夏回族自治区自然科学基金(NZ0958)
