摘要
目的探讨FK506缓释剂对大鼠坐骨神经再生纤维超微结构的影响。方法用梭形双通道桥接管桥接32只大鼠坐骨神经10mm缺损，根据梭形管的两支管内加入的药物不同将大鼠按随机数字表法分为：A组，12只，在两支管内均加入几丁糖凝胶；B组：12只，其中一侧支管内注入几丁糖＋FK506（B1），另一侧支管内注入几丁糖＋等渗盐水（B2）。于术后4，8，16周取再生神经的中段行透射电镜观察，并对8，16周再生神经的有髓和无髓纤维的面积、有髓纤维轴突直径、髓鞘厚度行计量分析。另取8只于术后12周进行电生理功能测定，并计算出神经传导速度。结果A组两支管内再生神经纤维的种类、数量差异无统计学意义。B1组再生纤维有髓和无髓纤维均增加，再生纤维面积较A组和B2组大（P〈0．05，0．01）；有骨髓纤维轴突直径、髓鞘厚度均较A组和B2组大（P〈0．05，0．01），术后12周。B1组神经传导速度较A组和B2组快（P〈0．05）。结论FK506可明显促进周围神经再生纤维超微结构的恢复。
Objective To discuss the effect of the sustained releasing agent FK506 on the uhra- structure of the regenerative nerve fibers. Methods The new fusiform-shaped double channel nerve conduit was used for bridge the sciatic nerve defects for 10 mm in 32 Sprague-Dawlcy rats. The rats were divided into Group A （100 pd chitin for both channels） and Group B （chitin plus FKS06 for B1 and chi- tin plus normal saline for B2） according to different addition of the drugs. At 8 and 12 week after opera- tion, the middle line of the regenerated nerve was observed under the transmission electron microscope. In the meantime, an analysis was done on the area of the regenerated nerve fibers （ myelinated and unmyeli- nated） , diameter and myelin thickness of the myelinated fiber axon at 8 and 16 months. Results There was not significant difference in aspects of type and number of the regenerative nerve fibers between two channels in Group A. However, the myelinated and unmyelinated fibers of the regenerative nerves were increased in Group B1, with larger area than Group B2 （ P 〈 0.05 ）. Conclusion FKS06 can significantly promote the regeneration of both the myelinated and unmyelinated fibers.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2011年第2期175-178,共4页
Chinese Journal of Trauma
基金
基金项目：中国博士后科学基金特别资助项目（20090263）
浙江省自然基金资助项目（G2005002）
关键词
他克莫司结合蛋白质类
周围神经
超微结构
Tacrolimus binding protenis
Peripheral nerve regeneration
Uhrastructure
Counting research
