摘要
利用紫外可见光谱和荧光光谱法研究三种黄酮类药物木犀草素、芹菜素和葛根素与牛血清白蛋白(BSA)在不同温度下(292 K和311 K)的相互作用。结果表明:三种黄酮类药物与牛血清白蛋白形成复合物导致牛血清白蛋白荧光猝灭,试验数据采用Stern-Volmer拟合方程处理及双对数方程处理,进一步证明结合反应引起的荧光猝灭属于静态猝灭。采用位点结合模型公式、热力学公式和F rster非辐射能量转移理论计算了结合常数、结合位点数及作用力类型以及结合距离。
Mechanism of the interaction between 3 flavonoids,i.e.,luteolin,apigenin and puerarin,with bovine serum albumin（BSA） under different temperatures（292 K and 311 K） was studied by UV-VIS spectrophotometry and fluorospectrometry.It was found that the formation of the complexes led to quenching of fluorescence of BSA,which was proved to be a process of static quenching by the fitting equations of Stern-Volmer and double logarithm equation.The binding constants,number of binding sites,types of binding force and binding distances were found by applying the equation of site-binding model,thermodynamic equations,and Frster theory of transfer of non-radiation energy.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2011年第2期186-190,共5页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
国家自然科学基金(20975081)
陕西省教育厅自然科学基金项目(2010JK899)
西北大学研究生交叉学科资助项目(07YJC09)
关键词
荧光光谱
荧光猝灭
牛血清白蛋白
木犀草素
芹菜素
葛根素
Fluorescence spectroscopy
Fluorescence quenching
Bovine serum albumin
Luteolin
Apigenin
Puerarin
