摘要
目的:建立猕猴桃根多糖脂质体包封率的测定方法。方法:采用阴离子交换树脂法分离脂质体和游离APPS,采用紫外-可见分光光度法测定游离APPS含量,计算脂质体包封率。结果:以纤维素DE-52阴离子交换树脂为填料,采用1.0mol·L-1NaCl溶液洗脱,游离APPS的回收率99.21%,空白脂质体的回收率0.07%;线性范围11.66～58.29 mg·L-1(r=0.999 4),日内和日间精密度均符合要求(RSD均小于2%),方法平均回收率99.28%,RSD 0.66%;APSP脂质体的平均包封率70.16%。结论:此法简便、可行,可用于猕猴桃根多糖脂质体包封率的测定。
Objective：The method was established to determine the entrapment efficiency for the APPS liposome.Method：Anion exchange resin was used to separate the liposome and free APPS;UV-visible spectrophotometry was used to determine the content of APPS,and liposome encapsulation efficiency was calculated.Result：The method established was as follows：the anion-exchange resin was used with 1.0 mol·L^-1 NaCl as eluent;the free APPS recovery was 99.21%,liposome recovery was 0.07% with the linear range of 11.66-58.29 mg·L^-1（r = 0.999 4）.The intra-and inter-day precisions（RSD） could meet the requirements（within 2.0%）;the average recovery was 99.28%,RSD was 0.66%.The encapsulation efficiency of APPS liposome was 70.16% on average.Conclusion：This method is simple and feasible,and can determine the entrapment efficiency for the APPS liposome.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第6期20-23,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
浙江省科技厅重点项目(2008C23068)
浙医江省中药管理局项目(2007CA114)
