摘要
在田间原位条件下,利用根系形态差异显著的自交系掖478和武312为亲本构建的BC4F3群体,采用改进的PLABQTL软件中的复合区间作图法对抽雄期(开花前10 d)和灌浆初期(开花后15 d)玉米根系性状的变化和地上部生物量进行QTL定位。并分析其遗传机制。结果表明,花前花后对根干重、总根长、侧根长、轴根长、轴根数等根系性状共检测出27个QTL,单个QTL贡献率为5.2%～15.7%,其中在染色体臂6.02和10.04区域同时检测到控制着地上部生物量、总根长、侧根长和轴根数等性状的QTLs,两个不同生育时期检测到的共同QTL共有8个。玉米花前花后控制根系生长的QTL因生长发育阶段不同而存在着特异性,而且对地上部生物量形成有重要贡献,这为了解田间条件下根系的生长发育和进一步进行遗传改良奠定了遗传基础。
The purpose of this experiment was to study the variation of maize root around flowering for fully understanding the genetic mechanism of root development under field conditions and to offer theory basis for genetic improvement of root in high yield and quality agricultural system. A BC4F3 population （187 lines） derived from Ye478 （with big root system） as the donor, and Wu312 （with small root system） as the recipient was developed. QTL for shoot dry weight （SDW） and root related traits in maize were identified by composite interval mapping of PLABMQTL software at the tasseling stage （VT stage, 10 days before silking） and early filling stage （R2 stage, 15 days after silking） under field conditions. Totally, 27 QTLs are detected for controlling root dry weight （ RDW）, total root length （TRL）, lateral root length （LRL）, axial root length （ARL） and axial root number （ARN）. The contribution of each QTL varies between 5.2%-15.7%. Two important regions are detected on chromosome bin 6.02 and 10.04 with controlled shoot dry weight, total root length, lateral root length and axial root number, and eight common QTLs are found at the both growth stages. There are specific genetic mechanisms for different root types at pre and post flowering stages, and the QTLs detected for root related traits are important to the formation of shoot growth of maize. These QTLs are helpful to understand the genetic basis of maize root under the field condition and offer the basis for further genetic improvement by MAS.
出处
《植物营养与肥料学报》
CAS
CSCD
北大核心
2011年第2期317-324,共8页
Journal of Plant Nutrition and Fertilizers
基金
国家自然科学基金(30600381)
国家基金创新群体项目(No.30821003)
公益性行业科研专项(200803030)资助
关键词
玉米
抽雄期
灌浆初期
根系
QTL
maize （Zea mays L. ）
tasseling stage
early filling stage
roots
quantitative trait loci （QTLs）
