摘要
目的:建立新的白术薄层色谱鉴别方法。方法:以苍术酮、白术内酯Ⅲ和白术内酯Ⅰ为对照品,分别以石油醚(60～90℃)与乙酸乙酯(10∶0.2)和正己烷-醋酸乙酯(3.5∶1)为展开剂,进行二次展开,10%硫酸乙醇显色后在波长365nm下检识。结果白术药材薄层色谱中,在与对照品色谱相应位置上显相同颜色的荧光斑点。结论该方法准确可靠,能够为白术的质量控制提供参考。
Objective： To establish the advanced TLC method of Atractylodes macrocephalae.Methods： Atractylon、Atraetylenolide Ⅲ and Atraetylenolide Ⅰ were used as the reference substance and the mixture of benzene（60~90℃）-ethyl acetate（50∶ 1） and hexane-ethylacetate（3.5∶ 1） as the mobile phase with twice-development,containing 10% sulfuric acid was used for coloration.The fluorescence chromatogram was detected at 365 nm in the ultraviolet lamp house.Results： The spots in the chromatogram of the test solution corresponding to the position to the each reference substance had the same color.Conclusion： The method is accurate and stable,and can be used for the quality control of Atractylodes macrocephalae.
出处
《中华中医药学刊》
CAS
2011年第3期535-536,共2页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省科技计划资助项目(2008R20007)
浙江省中医药科技计划资助项目(2007CB157)
关键词
白术
薄层色谱鉴别
苍术酮
白术内酯Ⅲ
白术内酯Ⅰ
Atractylodes macrocephalae
TLC
Atractylon
Atraetylenolide Ⅲ
Atraetylenolide Ⅰ
