摘要
采用超声波预处理固定化纤维素酶,通过单因素试验和响应面法探讨了超声波预处理条件(超声时间、超声频率、超声功率)以及预处理后的酶解温度和CMC-Na缓冲液pH值对固定化纤维素酶活性的影响,建立并分析了各因子与酶活相对关系的数学模型,优化得到的最佳条件为:酶解温度58.73℃、CMC-Na缓冲液pH值3.0、超声时间16.88 min、超声频率22.33 kHz、超声功率26.77 W,在此条件下,固定化纤维素酶活性与未加超声波预处理相比较提高了9.75%。
Immobilized cellulase was pretreated by ultrasound.Based on the single factor and response surface methodology,the effects of ultrasonic parameters,such as ultrasonic time,ultrasonic frequency and ultrasonic power,as well as enzymolytic temperature and pH value of CMC-Na buffer solution after pretreated were investigated.A mathematical model was established and analyzed to describe the relationships between the studied factors and enzyme activity.The optimum treatment parameters were as follows： enzymolytic temperature of 58.73℃,pH value of CMC-Na buffer solution of 3.0,ultrasonic time of 16.88min,ultrasonic frequency of 22.33kHz and ultrasonic power of 26.77W.Under the optimum conditions,enzyme activity of immobilized cellulose increased by 9.75%.
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2011年第3期150-155,共6页
Transactions of the Chinese Society for Agricultural Machinery
基金
江苏省自然科学基金资助项目(BK2008241)
江苏大学高级专业人才科研启动基金资助项目(08JDG003)
中国博士后科学基金资助项目(20090461072)
江苏省博士后科学基金资助项目(0902108C)
关键词
纤维素酶
酶固定化
超声波
响应面法
Cellulase
Immobilization of enzymes
Ultrasound
Response surface methodology
