摘要
为了明确生化处理和微生物降解的关系,通过增加耐盐菌的比例可以提高农药废水生化处理效果。从农药厂废水中分离到1株耐盐性毒死蜱降解菌——蜡状芽孢杆菌(Bacillus cereus HY-1),以从该菌中提取到的降解酶比活力为指标,进行产酶培养基和发酵条件的优化研究。通过单一因素试验和正交试验,对细菌HY-1的产酸培养基和发酵条件进行了优化。运用SPSS软件进行结果分析,所获优化培养基配方为:葡萄糖6.0 g/L,胰蛋白胨2.2 g/L,K2HPO4 2.0 g/L,KH2PO4 0.2 g/L,MgSO4.7H2O 0.1 g/L,NaCl 0.1 g/L和微量元素溶液2 mL/L。得到菌株发酵培养的最佳优化条件为:种子液培养时间为16 h,发酵培养时间为18 h,接种量为1%(V/V),发酵培养基初始pH值为7.0。氯化钠浓度为0?30 g/L时降解酶比活力不受影响,这是已报道的耐盐性最强的一株毒死蜱降解菌。
In order to improve the treatment effect of wastewater from pesticide enterprise,it is neces-sary to study the salt-tolerant characteristics and fermentation conditions of the degrading bacterium.A chlorpyrifos-degrading bacterium Bacillus cereus HY-1 was isolated in laboratory from pesticide wastewater.The optimal fermentation medium and fermentation conditions of HY-1 were selected by single factor and orthogonal test,and the amount of enzyme produced by HY-1 was estimated based on the specific activity of the enzyme.The experimental results were analyzed by the SPSS system.The optimal composition of the culture medium included glucose 6.0 g/L,tryptone 2.2 g/L,KH2PO4 2.0 g/L,K2HPO4 0.2 g/L,MgSO4·7H2O 0.1 g/L,NaCl 0.1 g/L,trace elements solution 2 mL/L.The optimal fermentation conditions of HY-1 were that incubation time of the seed culture was 16 h,fermentation time was 18 h,inoculum amount was 1%（V/V）,initial pH 7.0.The specific activity of the degrading enzyme was not affected while the sodium chloride concentration of the fermentation medium in-creased from 0 g/L to 30 g/L.To the best of our knowledge,this is the most extremely salt-tolerant bacterium reported that could degrade chlorpyrifos.
出处
《微生物学通报》
CAS
CSCD
北大核心
2011年第3期355-361,共7页
Microbiology China
基金
国家高技术研究发展计划(No.2007AA10Z404)
关键词
毒死蜱
降解酶
培养基
发酵条件优化
耐盐菌
Chlorpyrifos
Degrading enzyme
Culture medium
Fermentation condition optimization
Salt-tolerant bacterium
