摘要
目的:在单因素实验的基础上,利用表面响应曲面法(RSM)对HPLC-UV法测定牛磺罗定含量中的牛磺罗定衍生化条件进行优化。方法:以衍生化试剂不同的浓度、反应时间和反应温度为因子,以反应后衍生化产物的量(用峰面积表示)为响应因子,对衍生化条件进行优化。结果:最佳衍生化条件是反应温度为100℃,衍生化试剂浓度为20 g.L-1,反应时间为88 min。结论:本方法结果准确可靠,可应用于牛磺罗定的衍生化。
Objective：On the base of the single factor investigation,response surface mathodology was applied to optimize the derivatization condition of taurolidine.Methods：Different derivatization reagent concentrations,reaction time and reaction temperature were taken as factor.The amount of the derivative products（expressed by peak area）was taken as response factor.Results：The optimizing derivatization condition of taurolidine was as follows：the reaction temperature（100 ℃）,the derivatization reagent concentration（20 g·L-1）,the reaction time（88 min）.Conclusion：The method is accurate,reliable and can be applied to derivatize taurodiline.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第3期479-483,共5页
Chinese Journal of Pharmaceutical Analysis
关键词
曲面法
牛磺罗定
对甲基苯磺酰氯
衍生化
response surface methodology
taurolidine
p-toluenesulfonyl chloride
derivatization
