摘要
目的:建立梯度洗脱的HPLC法测定硫普罗宁注射液中有关物质。方法:使用C18色谱柱,以0.1 mol.L-1磷酸二氢钾溶液(用磷酸调节pH至3.2)-甲醇为流动相,梯度洗脱,流速1.0 mL.min-1,检测波长210 nm。结果:本法可快速有效分离硫普罗宁注射液中各有关物质。结论:本方法灵敏、准确,专属性强。
Objective：To establish a method for the determination of related substances in tiopronin injection by using gradient elution of HPLC. Methods：C18 column was adopted;Mobile phase consisted of buffer（pH 3.2） – methanol with gradient elution at a flow rate of 1.0 mL·min-1,and the detection wavelength was 210 nm. Results：Tiopronin and its related substance were oboviously separated under the chromatographic system decribed above. Conclusion：The method is sensitive,accurate and specific.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第3期499-503,共5页
Chinese Journal of Pharmaceutical Analysis
