摘要
目的探讨盐酸普鲁卡因对人结肠癌HT-29细胞生长的抑制作用及其机制。方法应用不同浓度（0-10mmol/L）盐酸普鲁卡因处理HT-29细胞,通过倒置显微镜观察细胞形态学改变、四甲基偶氮唑盐比色法（MTT）检测细胞存活率、流式细胞术（FCM）观察细胞周期变化。结果倒置显微镜观察盐酸普鲁卡因处理后的HT-29细胞呈现缩小、皱缩、空泡、脱壁等增殖变慢的形态学特征;MTT结果分析表明盐酸普鲁卡因能显著抑制HT-29细胞增殖,其抑制率呈药物浓度及时间依赖性;FCM结果显示盐酸普鲁卡因可以使HT-29细胞的G0/G1期延长,S期缩短。结论盐酸普鲁卡因能够使HT-29细胞的生长阻滞在G0/G1期,从而显著抑制HT-29细胞的增殖,并且具有剂量、时间依赖效应。
Objective To investigate the influence of Procaine hydrochloride（PCA） on human colon cancer HT-29 cells proliferation and explore its mechanism.Methods HT-29 cells were treated with 0~10mmol/L of PCA,cell morphology changes were observed under contrast microscope.Cell proliferation was detected by MTT assay and cell cycle was detected by fIow cytometry（FCM）.Results With different concentrations of PCA being used to dispose cancer cells separately,growth inhibition taking the form of cell＇s decreased size,shrinkage,vacuoles,cells abscised from the inner surface of the bottle became increasing obvious under contrast microscope.With the rise of the PCA concentration,HT-29 cells has a presentation of negative acceleration in the proliferation by MTT.The analysis of the result indicated that PCA can inhibit the cell proliferation significantly whose effect was replying on the dosage and time.The result by FCM shows that PCA can lengthen the G0/G1 phase and shorten the S phase in HT-29 cells.Conclusion PCA can make the growth of the HT-29 cells blocked in G0/G1 phase,thus inhibiting the cell multiplication significantly,whose effect was relying on the dosage and time.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2011年第2期137-140,共4页
Cancer Research on Prevention and Treatment
基金
三峡大学研究生科研创新基金资助项目(2009-35)
