摘要
目的探索帕瑞昔布联合右颈交感神经干离断(TCST)对大鼠心肌梗死后左室重构的影响。方法成年雄性SD大鼠40只,采用结扎左冠状动脉前降支方法制备急性心肌梗死模型,制模成功后,采用右TCST治疗和(或)给予帕瑞昔布8 mg·kg^(-1)·d^(-1)治疗。于建模处置4wk后行右颈总动脉插管测定左心室收缩压(LVSP)、左心室舒张末期压(LVEDP)、左心室收缩压上升最大速率(+dp/dt_(max))和左心室收缩压下降最大速率(-dp/dt_(max));放射免疫法检测血浆血栓素A_2(TXA_2)和前列腺素I_2(PGI_2)的浓度;体视学三维形态定量分析左室重构,测定左室心肌细胞体积密度Vv、心肌组织总体积Vt、心肌细胞总体积Vc。结果心肌梗死制模后各组LVSP、±dp/dt_(max)明显降低,而LVEDP升高(均P<0.01),PGI_2、PGI_2/TXA_2显著降低、TXA_2增加(P<0.01),Vv、Vt、Vc均明显升高(P<0.01);右TCST或帕瑞昔布治疗后LVSP及±dp/dt_(max)升高(P<0.01或P<0.05)而LVEDP降低,PGI_2、PGI_2/TXA_2显著增加而TXA_2降低,Vv、Vt、Vc均降低(P<0.01);右TCST联用帕瑞昔布后心功能改善更显著,Vv、Vt、Vc降低更明显(P<0.05)。结论帕瑞昔布与右TCST单用及合用均能显著提高心肌舒缩功能,调节PGI_2与TXA_2的相对平衡,延缓急性心肌梗死大鼠的左室重构过程,但两者合用效果更佳。
AIM To investigate the effects of parecoxib combined with transection of right cervical sympathetic trunk （TCST） on left ventricular remodeling （LVRM） after myocardial infarction in rats. METHODS Forty adult male SD rats （240 ± 10） g were randomly divided into five groups. Rat model of acute myocardial infarction （AMI） was made by ligation of the left anterior descending branch （LAD） of the coronary artery in 32 rats. Eight sham-operated animals were used as controls （group S） . AMI rats in group T1 were treated with right TCST, in group T2 were administered with pareeoxib （8 mg·kg^-1 ·d^-1） , in group T3 were treated with parecoxib （8 mg ·kg^-1 ·d^-1） combined with right TCST, and in group C were treated with the separation of right cervical sympathetic trunk. Four weeks after the operation, LVSP, LVEDP, ＋ dp/dtmax were measured by the right common carotid artery catheterization. TXA2 and PGI2 were determined by RIA. Left ventricular myocardium volume （Vt） , the volume density （Vv） of myocardial cells, myocardial cell volume （Vc） were quantitatively analyzed by stereological methods. RESULTS Compared with group S, LVSP, ＋ dp/ dtmax and PGI2/TXA2 decreased （P 〈 0.01）, while LVEDP, TXA2, Vv, Vt, and Vc increased （P 〈 0.01） in myocardial infarction groups. Compared with group C, LVSP, ± dp/dtmax, PGI2 and PGI2/TXA2 increased （P 〈 0.01 or P 〈 0.05）, while LVEDP, TXA2, Vv, Vt and Vc decreased （P 〈 0.01 or P 〈 0.05） in group T1 and T2. Myocardial function improved remarkably, while Vv, Vt and Vc decreased significantly （P 〈 0.05） in group T3. CONCLUSION Parecoxib combined with right TCST is effective in improving the myocardial function, regulating PGI2/TXA2, mitigating the process of ventricular remodeling after myocardial infarction, and is superior to single therapy.
出处
《中国新药与临床杂志》
CAS
CSCD
北大核心
2011年第2期122-125,共4页
Chinese Journal of New Drugs and Clinical Remedies
