摘要
目的:应用激光诱导荧光技术检测光动力治疗大鼠牙周炎过程中荧光光谱强度,分析治疗的最佳激光功率和治疗时间。方法:把20只大鼠上颌第一磨牙用细菌结扎线建立牙周病模型,随机分成5组,每只大鼠左侧进行治疗,右侧不进行治疗(阴性对照)。每组大鼠使用亚甲基蓝光敏剂,在激光波长660nm特定功率下进行光动力治疗。利用光学多通道分析仪采集治疗过程中产生的荧光光谱强度,并观察治疗后各组的细菌菌落数。结果:使用激光功率250mW,连续照射时间4min时,产生的荧光光谱强度最大。治疗后检测发现该组细菌菌落数减少最多,验证了在该条件下治疗达到了最优效果。结论:激光诱导荧光法监测光动力治疗组织内光敏剂荧光变化可有效地反映光敏剂含量的变化,对于了解光动力反应的进行情况以及预测治疗效果具有一定指导意义,为制定光动力治疗慢性牙周炎方案提供理论依据。
Objective： To monitor the fluorescence spectra intensity by laser induced fluorescence method during photodynamic therapy （PDT） in the treatment for periodontitis in rats and analyze the optimal laser power and therapy time. Methods： Periodontitis was developed by submerging ligatures at the subgingival region of maxillary molars in 20 rats. Five groups were divided randomly and the left part of each rat was treated by PDT and the right part was kept （as the negative control）. Each group was treated with methylene blue plus 660nm laser power. The fluorescence spectra intensity was measured and analyzed. The bacterial flora of each group was observed after therapy. Results： The fluorescence spectra intensity is maximal when the excited laser power is 250mW and the continue therapy time is 4 min. Under these conditions, the significant reduction of total bacterial flora was achieved and the therapeutic effect is best. Conclusion： Laser induced-fluorescence method established in this study can provide an efficient way to reflect the photosensitizer concentration by monitoring the fluorescence change. It will be helpful to understand the therapeutic theory and efficiency of PDT and provide the optimal therapeutic schedule.
出处
《应用激光》
CSCD
北大核心
2011年第1期89-91,共3页
Applied Laser
关键词
医用光学与生物技术
激光诱导荧光
光动力疗法
牙周炎
荧光光谱
medical optics and biotechnology
laserinduced-fluorescence
photodynamictherapy
periodontitis~ fluorescence spectra
