摘要
目的观察牛膝醇提取物对去卵巢骨质疏松大鼠骨密度(BMD)、骨转换和骨组织Ⅰ型胶原蛋白表达的影响。方法背侧切口切除大鼠双侧卵巢,分为去卵巢组,牛膝提取物高、中、低剂量组和雌激素对照组,另设一假手术组,分别给予基础饲料和不同剂量治疗药物,12w后使用双能X线骨密度仪测量股骨、胫骨和腰椎的骨密度;采用酶联免疫吸附法对骨转换指标血清骨碱性磷酸酶(BAP)、血清骨钙素(BGP)、抗酒石酸酸性磷酸酶(TRACP)、尿Ⅰ型胶原氨基末端肽(NTx)/肌酐比值(NTx/Cr)进行测定,并利用Western免疫印迹法检测骨组织Ⅰ型胶原蛋白表达的变化。结果大鼠去势后骨密度和Ⅰ型胶原蛋白的表达显著下降(P<0.01),骨转换指标有较大变化。牛膝醇提取物高、中剂量组可使去势大鼠骨密度和Ⅰ型胶原蛋白的表达显著提高,改善骨转换指标的变化,且存在一定的剂量-效应关系。结论牛膝醇提取物能提高去势大鼠的骨密度和Ⅰ型胶原蛋白的表达。
Objective To investigate the effects of achyranthes bidentata BI extracts on bone mineral density （BMD） , bone turnover rate and the expression of type I collagen in bone of ovariectomized （OVX） rats. Methods Rats with bilateral removal of ovaries were divided into OVX control group,low-,middle-, and high-dose of achyranthes bidentata BI extracts group, estrogen control group,and sham operation group. Basic feed and different dose of curative materials were given. After 12 weeks,BMDs of the femur, the tibia, and the lumbar vertebra were measured using DXA. Serum bone alkaline phosphates （BAP） , bone glaprotein （BGP）, tartrate-resistant acid phosphatase （TRACP） activity, and urine deoxypyridinoline/ creatinine （UDpd/Cr） were measured with enzyme-linked immuosorbent assay. The amount of type I collagen protein in bone tissue were detected with Western blot. Results BMD and the expression of type I collagen in the bone significantly decreased and bone turnover rate changed obviously in OVX group. High- and middle-dose of achyranthes bidentata BI extracts increased BMD and type I collagen in OVX rats significantly, and improved the bone turnover rate, in a dosb-dependent manner. Conclusion Achyranthes bidentata BI extracts was able to elevate BMD and improved the expression of type I collagen protein in OVX rats.
出处
《中国骨质疏松杂志》
CAS
CSCD
2011年第2期109-112,共4页
Chinese Journal of Osteoporosis
关键词
牛膝
骨质疏松
骨密度
胶原
Achyranthes bidentata BI.
Osteoporosis
Bone mineral density
Collagen
