摘要
目的研究重组pCKM—mPTH质粒在SD大鼠甲状旁腺功能低下症模型治疗中对血钙浓度的影响。方法将60只SD大鼠制作甲状旁腺功能低下症模型后，随机分为3组，分别肌注pcDNA3．1（＋）空质粒、mPTH质粒和pCKM—mPTH质粒，不同时间用放免法测定血清甲状旁腺素（PTH）浓度，采用MTB比色法检测血清钙浓度。结果动物模型在注射mPTH和pCKM～mPTH质粒后，其血清PTH和钙浓度均出现显著升高，之后出现回落，但pCKM—mPTH质粒组回落较缓慢，其作用维持近30天。结论质粒pCKM—mPTH在SD大鼠体内合成的甲状旁腺素能有效的调节血清钙的浓度，且转染效率和维持时间优于不含pCKM启动子的mPTH质粒。
Objective To study the influence of recombinant plasmid pCKM-mPTH to the serum calcium concentration of the SD rats ofhypoparathyroidism model. Methods 60 SD rats of hypoparathyroidism models were randomly divided into three groups, which were injected intramuscular pcDNA3.1 （ ＋ ） empty vector, mPFH plasmids and pCKM-mPTH plasmid via muscle. At different times, rats were measured serum parathyroid hormone （ PTH ） concentration using radioimmunoassay, and serum calcium concentration using MTB colorimetric assay. Results After being injected with mPTH and pCKM-mPTH plasmid, serum PTH and calcium appeared significantly increased and fell later, but that of pCKM-mPTH plasmid groups fell more slowly, which continued nearly 30 days. Conclusion Recombinant plasmid pCKM-mPTH can effectively regulate the concentration of serum calcium after transfecting SD rats of hypoparathyroidism model. Furthermore, its transfection efficiency and the maintenance of time are superior to non-pCKM promoter mPTH plasmid.
出处
《国际医药卫生导报》
2011年第6期657-660,共4页
International Medicine and Health Guidance News
基金
中山大学青年教师基金资助项目（3171911）
