摘要
目的培养大鼠胚胎神经干细胞,诱导分化获得较高纯度的少突胶质细胞。方法取孕11.5 d的胚胎大鼠神经管尾段制备单细胞悬液,无血清条件培养基培养,获得神经干细胞;加入胎牛血清诱导分化,利用神经元、星形胶质细胞和少突胶质细胞不同的生长特性进行分离,获取较高纯度的少突胶质细胞。结果本实验得到纯度达90%以上的少突胶质细胞。结论利用培养大鼠胚胎神经干细胞诱导分化可以获取较多较高纯度的少突胶质细胞,以进行进一步研究。
Objective To culture embryonic neural stem cells of rats and differentiate into oligodendrocytes in vitro,purifing in order to obtain highly purified oligodendrocyte.Methods The cells derived from the posterior segment of neural tube in E11.5d rat were cultured in serum-free conditioned medium and neural stem cells were obtained,which were induced to differentiate into neurons,astrocytes and oligodendrocyte by adding fetal bovine serum.To separate these differentiated cells according to their different physical and chemical characteristics,highly purified oligodendrocytes were gained.Results The purity of oligodendrocytes can be more than 90%.Conclusion Large quantities and high purity of oligodendrocytes were gained by culturing embryonic neural stem cells of rats in vitro,for the purpose of further research and analysis.
出处
《滨州医学院学报》
2011年第1期26-28,32,共4页
Journal of Binzhou Medical University
基金
山东省优秀中青年科学家奖励基金(2004BS02017)
关键词
神经干细胞
少突胶质细胞
培养
分化
纯化
neural stem cells
oligodendrocyte cell
culture
identification
purification
