摘要
[目的]简化甘蔗试管苗的培养程序。[方法]用添加不同浓度赤霉素的MS培养基培养ROC22甘蔗试管苗,测定试管苗株高、节数量、移栽存活率等指标。[结果]赤霉素处理对试管苗的株高、节数量、移栽存活率和生根有促进作用,特别是对试管苗的株高、节的生长有显著促进作用,以赤霉素处理浓度1~3 mg/L为最好。[结论]赤霉素能显著地促进ROC22试管苗的瓶外生根率和存活率,因此甘蔗试管苗可以实现生根与驯化移栽一步完成。
[Objective]The purpose of this experiment is to simplify the sugarcane tissue culture procedure from in vitro rooting to nursery management.[Method]Micropropagated ROC22 shoots are cultured on MS medium supplemented with different concentration of Gibberellins.Then the height,quantity of nodes and survival rate of transplants are investigated.[Result]The results show that Gibberellins is beneficial to stem elongation,node development,higher survival rate of transplants and root system development,especially useful for the stem elongation,node growth and higher survival rate of transplants.The best concentration of Gibberellins treatment is in the range of 1-3 mg/L.[Conclusion]Gibberellins treatment can significantly improve the ex vitro rooting and survival rate of transplants,which indicates that the acclimation and rooting of sugarcane micropropagated shoots could be integrated together in the nursery.
出处
《安徽农业科学》
CAS
北大核心
2011年第4期2059-2060,共2页
Journal of Anhui Agricultural Sciences
基金
广西自然科学基金项目"甘蔗试管苗瓶外生根技术研究"(桂科回0991012)
广西甘蔗所基本科研业务项目"甘蔗试管苗瓶外生根技术研究"(G2009013)
国家支撑计划课题"高产高效甘蔗良种繁育与栽培技术研究与示范"(2007BAD30B04)
关键词
甘蔗试管苗
赤霉素
瓶外生根
Sugarcane micropropagated shoots
Gibberellins
Ex vitro rooting
