摘要
目的建立实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomyelitis,EAE)大鼠模型,观察其组织病理特征。方法 采用豚鼠全脊髓匀浆制备抗原免疫Lewis大鼠建立EAE模型。每天监测动物体质量和行为学变化。应用HE染色观察脑和脊髓炎性浸润、血管袖套等变化,罗克沙尔坚牢蓝(luxol fast blue,LFB)染色观察脊髓脱髓鞘变化,电子显微镜观察脊髓髓鞘超微结构。结果 随着病程进展,EAE模型组大鼠体质量下降明显;从第8天起出现行为学异常,神经功能损伤评分逐渐增高,于第12天达峰值。病理形态学检查显示,EAE模型组大鼠脑组织血管周围和脑实质有大量炎性细胞浸润;脊髓腰膨大脊膜出现炎性水肿,并有炎性细胞浸润;脊髓血管周围有大量炎性细胞密集环绕,呈"袖套样"改变;脊髓髓鞘大面积脱失,且髓鞘板层松散。结论 采用该模型作为急性MS的研究模型较为理想。
Objective：To develop an experimental autoimmune encephalomyelitis（EAE） model in Lewis rats and observe its pathological characteristics for studying multiple sclerosis.Methods：An EAE model was established by immunizing Lewis rats with both guinea pig spinal cord homogenate in complete Freund＇s adjuvant and pertussis toxin.The body weight and behavioral change of rats were monitored each day.Hematoxylin eosin（HE） staining was used to observe the inflammatory infiltration in the brain and spinal cord tissue,luxol fast blue（LFB） staining was utilized to detect demyelination change,and electron microscope was used to observe the ultrastructure of myelin sheath in the spinal cord.Results：Along with the progression of course of disease,EAE model rats showed a significant decrease of body weight;the behavioral abnormality appeared on day 8,the score of nervous function increased gradually,and reached peak on day 12.HE and LFB staining showed the typical pathological changes including perivascular inflammatory cell infiltration and demyelination in white matter.The layers of myelin sheath turned looser in the spinal cord of EAE rats.Conclusion：An EAE model was successfully established in Lewis rats by using whole guinea pigs homogenate.
出处
《首都医科大学学报》
CAS
北大核心
2011年第1期84-89,共6页
Journal of Capital Medical University
基金
国家自然科学基金项目(81001656
30973513
30873230)
北京市自然科学基金项目(7092014)~~
