摘要
目的：以白细胞介素1β（IL-1β）和胶质源性神经营养因子（GDNF）为诱导剂,探索小鼠胚胎中脑神经干细胞（M-NSCs）向多巴胺能神经元的的分化.为M-NSCs移植治疗帕金森病（PD）提供实验依据.方法： 在有血清条件下体外培养鼠胚M-NSCs,予以IL-1β和GDNF作诱导分化.酪氨酸羟化酶（TH）免疫细胞化学鉴定.流式细胞术检测TH阳性神经元的百分率.结果： GDNF组促进M-NSCs分化为TH阳性神经元的比例为13.53%,IL-1β组为9.66%,GDNF＋IL-1β联合培养组为16.22%,空白对照组仅3.46%,差异有统计学意义.结论： IL-1β和GDNF可明显促进M-NSCs分化成多巴胺能神经元.
Objective： To explore mouse embryonic neural stem cells to dopaminergic neurons division induced by IL-1β and GDNF, and provide the experimental basis for the mesencephalic neural stem cells （M-NSCs） transplantation for treatment of Parkinson＇s disease. Methods： M-NSCs were cultured and proliferated, and then IL-1β and GDNF were used to induce differentiation in the condition of fetal bovine serum. Immunocytochemical techniques were used to detect the TH-ir cells from M-NSCs differentiation, and Flow cytometry was used to detect the percentage of TH-ir in all the cells. Results： The proportions of M-NSCs differentiation into TH positive cells were as follows： 13.53% in the GDNF group, 9.66% in the IL-1β group, 16.22% in the GDNF＋IL-1β group. The data of each group were much higher than those in the blank control group （3.46%）. The proportions of NSCs differentiation into TH positive cells showed significant difference among experi- mental groups. Conclusion： IL-1β and GDNF can significantly increase the differentiation of dopaminergic neurons.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2011年第1期41-44,共4页
Chinese Journal of Anatomy
