摘要
目的应用激光多普勒血流探测仪（LDF）和激光多普勒血流成像仪（LDPI）监测Lewis大鼠后肢急性缺血模型血流和血压的动态变化,探讨大鼠后肢急性缺血后血流变化特点。方法切除大鼠左后肢股动脉制备急性后肢缺血模型,于术后2、7、14、28及49 d对手术侧和非手术侧肢体采用LDF进行血流、血压检测,于术后7 d采用LDPI进行血流检测。结果所有大鼠术后均成活,未发现后肢坏死;在术后14 d内手术侧后肢平均分为2分,在49 d平均分为1分。大鼠手术侧和非手术侧肢体血流比值在术后2 d由术前的1上升至1.31±0.439（P=0.021）,术后7 d和14 d分别为0.82±0.538和0.93±0.294,两者比较差异无统计学意义（P=0.502）,但均明显低于术后2 d的值（P=0.032和P=0.019）;术后28 d下降到最低点（0.41±1.970）,明显低于术后2、7和14 d值（P=0.004、P=0.007和P=0.006）;在术后49 d手术侧后肢血流恢复到接近术前值（0.98±0.093）,明显低于术后2 d（P=0.010）而高于术后28 d值（P=0.005）,与术后7 d和14 d的差异无统计学意义（P=0.126和P=0.382）。大鼠手术侧和非手术侧肢体血压比值术后2 d由术前的1明显下降至0.47±0.375（P=0.031）;术后7 d继续下降至0.44±0.118,与术后2 d比较差异无统计学意义（P=0.203）;术后14 d下降到最低点（0.35±0.115）,明显低于术后2 d和7 d值（P=0.001和P=0.036）;术后28 d开始上升（0.54±0.146）,明显高于术后14 d值（P=0.008）,但与术后2 d（P=0.493）和7 d（P=0.551）的差异无统计学意义;术后49 d恢复接近术前值（0.97±0.094）,明显高于术后2、7、14和28 d值（P=0.013、P=0.021、P=0.002和P=0.031）。结论切除大鼠后肢股动脉及分支的方法制备后肢急性缺血模型,在术后14~28 d患肢缺血处于最严重阶段。LDF和LDPI可以动态监测肢体血流及血压的变化,对监测大鼠后肢术后缺血的动态演变过程有着重要的作用。
Objective To evaluate the dynamic changes of blood flow and blood pressure of acute hindlimb ischemia of rats by laser Doppler flowmetry（LDF） and laser Doppler perfusion imaging（LDPI）.Methods The acute hindlimb ischemia model of rats was established by resection of rats femoral arteries of left hindlimb.The blood flow and blood pressure between operated and nonoperated hindlimbs were examined by LDF on 2,7,14,28,and 49 d after operation.And the blood flow was evaluated by LDPI on 7 d after operation.Results All rats survived after operation and no hindlimb necrosis occurred.The mean score was 2 on 14 d after operation and 1 on 49 d after operation.The ratio of blood flow between operated and nonoperated hindlimbs on 2 d after operation significantly increased from 1 to 1.31±0.439（P=0.021）.The ratio of blood flow on 7 d（0.82±0.538） and 14 d（0.93±0.294） after operation was significantly lower than that on 2 d after operation（P=0.032 and P=0.019）,although the difference between the two former was not significant（P=0.502）.Furthermore,the ratio of blood flow on 28 d after operation reached the bottom（0.41±1.970）,which was obviously lower than that on 2,7,and 14 d after operation（P=0.004,P=0.007,and P=0.006）.The blood flow of operated hindlimbs recovered approximately the value before operation（0.98±0.093）,which was significantly lower than that on 2 d（P=0.010）,higher than that on 28 d（P=0.005）,but not different from that on 7 d and 14 d after operation（P=0.126 and P=0.382）.The ratio of blood pressure between operated and nonoperated hindlimbs on 2 d after operation significantly increased from 1 to 0.47±0.375（P=0.031）.The ratio of blood pressure decreased on 7 d after operation（0.44±0.118）,which was not different from that on 2 d after operation（P=0.203）.Furthermore,the ratio of blood pressure on 14 d after operation reached the bottom（0.35±0.115）,which was obviously lower than that on 2 d and 7 d after operation（P=0.001 and P=0.036）.On 28 d after operation,the ratio of blood pressure increased（0.54±0.146）,which was significantly higher than that on 14 d after operation（P=0.008）,while not different from that on 2 d（P=0.493） and 7 d after operation（P=0.551）.The ratio of blood pressure recovered approximately the value before operation（0.97±0.094）,which was significantly higher than that on 2,7,14,and 28 d（P=0.013,P=0.021,P=0.002,and P=0.031）.Conclusion Acute hindlimb ischemia model of rats can be established by resection of rats femoral arteries of left hindlimb and the most serious stage of hindlimb ischemia is on 14-28 d after operation.LDF and LDPI are of importance for monitoring the dynamic changes of rats hindlimb ischemia after operation.
出处
《中国普外基础与临床杂志》
CAS
2011年第2期137-141,共5页
Chinese Journal of Bases and Clinics In General Surgery
基金
首都医科大学基础临床合作课题(项目编号:2006JK62)~~
