摘要
【目的】研究豆壳过氧化物酶(SBP)-H2O2-KI三元体系杀菌作用及其可能的杀菌机制。【方法】以细菌生长的浊度(OD600)为指标检测SBP-H2O2-KI体系的抑菌作用;以活细胞计数(CFU)为指标检测SBP-H2O2-KI体系的杀菌作用;以最低抑菌浓度(MIC)为指标检测亚致死剂量的SBP-H2O2-KI体系作用下连续传代细菌的敏感性变化;以物理和化学方法检测SBP-H2O2-KI体系中活性氧基团等功能基团的生成与否,以期解释SBP-H2O2-KI体系的杀菌机制。【结果】SBP-H2O2-KI体系对多种细菌有高效、快速杀菌作用,作用时间仅为几分钟。在亚致死剂量浓度下连续培养的细菌悬液对体系的耐受能力(MIC)没有显著性变化,从中也不能分离到抗性/耐性突变株。物理和化学方法检测结果表明SBP-H2O2-KI反应过程中无羟基自由基产生;化学方法检测结果表明SBP-H2O2-KI反应过程中无超氧阴离子自由基产生;而有单线态氧和单质碘的产生。【结论】根据实验结果可以推测:SBP-H2O2-KI体系的杀菌力可能主要来源于单线态氧和碘的活性中间态,而不是羟基自由基和超氧阴离子。此外,SBP-H2O2-KI体系所具备的高效、快速的杀菌作用,以及细菌对其不易产生抗性的特点,预示此体系在医疗以及植保等方面有广泛的应用前景。
[Objective]To study the bactericidal effect and the possible mechanisms of the three components system [soybean peroxidases（SBP）-hydrogen peroxide（H2O2）-potassium iodide（KI）,SBP-H2O2-KI].[Methods] The inhibition and bactericidal effect of SBP-H2O2-KI system to bacteria was detected by OD600 and the number of live bacteria（CFU）.The sensitivity was tested by comparing the minimum inhibitory concentration（MIC） of bacterial cultures before and after cultured under sub-lethal dose of SBP-H2O2-KI system.Oxidizing activity groups were detected with physical and chemical methods in order to explain the bactericidal mechanisms of SBP-H2O2-KI system.[Results] SBP-H2O2-KI ternary system had rapid and high efficient bactericidal effect to a variety of bacterial strains in just several minutes.The MICs had no significant changes when bacterial cultures continuously cultured in sub-lethal dose of SBP-H2O2-KI system,and no resistance / tolerance mutant strains could be isolated from them.Both physical and chemical test results showed that no hydroxyl radical produced in SBP-H2O2-KI reaction system,chemical test results showed that no superoxide anion but a singlet oxygen and iodine produced in SBP-H2O2-KI reaction system.[Conclusion]These results suggested that singlet oxygen and iodine or the iodine intermediate state may possible be the main sterilization factors for SBP-H2O2-KI system,and hydroxyl radical and superoxide anion not.In addition,the both characteristics of SBP-H2O2-KI system： rapid and high efficient bactericidal effect,and bacteria difficultly resisting to it,indicated it would have a good potential application in medical and plant protection area.
出处
《微生物学报》
CAS
CSCD
北大核心
2011年第3期393-401,共9页
Acta Microbiologica Sinica
基金
国家自然科学基金(81041089)
山东省自然科学基金(ZR2009BM020,Y2007D35)~~
