摘要
[目的]研究椎间盘内注射转化生长因子-β1(transforming growth factor-β1,TGF-β1)对兔退变腰椎间盘软骨终板Ⅱ型胶原的影响,以探讨局部注射TGF-β1对防治软骨终板退变的作用。[方法]选用6个月龄日本大耳白兔46只,雌雄不限,体重为(2.5±0.2)kg,随机分为对照组、预防组和治疗组。对照组18只,预防组18只,治疗组10只。所有实验动物均通过切开皮肤及皮下组织,剥离骶棘肌、切断L5、6、L6、7棘上、棘间韧带、两侧关节突关节囊及关节后外1/2造成L5、6、L6、7椎间失稳模型。预防组动物在完成椎间失稳手术后立即于同一切口通过侧后方入路显露L5、6、L6、7椎间盘,并在椎间盘内注射TGF-β1。对照组及预防组分别于椎间失稳术后3、6个月各8只取材。治疗组于椎间失稳术后第3个月再行侧后方入路L5、6、L6、7椎间盘内注射TGF-β1,第6个月取材。运用免疫组织化学方法对标本进行Ⅱ型胶原测定,并用SPSS 15.0软件进行统计学分析。[结果]①术后3、6个月预防组软骨终板内Ⅱ型胶原与对照组相比有高表达,差异有显著统计学意义;②术后第6个月治疗组软骨终板内Ⅱ型胶原与对照组相比有较高表达,差异有显著统计学意义。[结论]在兔椎间盘内注射TGF-β1可以提高Ⅱ型胶原的表达,具有延缓软骨终板退变的作用。
[Objective]To study the effects of transforming growth factors-β1 injection on collagen Ⅱ in the degenerated cartilage endplate of rabbits,and to explore the function of preventing and treating degenerated cartilage endplate by injecting TGF-β1 locally. [Methods]Forty-six six-month white rabbits from Japan were choosen,regardless of genger,weighing（2.5~0.2） kg,and were divided three groups at random：contrast group,prevention group and treatment group.Eighteen rabbit were distrbuted into contrast group,another 18 into the second group,the rest of 10 into the treatment group.All the rabbits＇ skins,subcutaneous tissues,L5、6、L6、7,interspinal ligaments,bilateral articular projections and articular cysts were cut,leaving intervertebral destabilization between L5、6 and L6、7.The rabbits in the prevention group were injected TGF-β1 and L5、6 and L6、7 intervertebral plates were exposed through lateral back opening after the operation and given TGF-β1 injection.The rabbits in the other groups were experimented after three months and eight months.Treatment group did the same in the third month and taken samples in the sixth month.Collagen Ⅱ was measured using immunohistochemical method and performing statistic analysis with SPSS 15.0 statistic soft ware.[Results]①The level of collagen Ⅱ in the cartilage endplate had greatly risen in the rabbits after operation for three months and six months in the contrast group significantly,with significant difference.②High expression of level of collagen Ⅱ appearred in the treatment group compared with contrast group.[Conclusion]Injection of TGF-β1 into rabbits＇ intervertebral plates can raise the level or expression of collagen Ⅱ,and prevent slow cartilage plates from degenerating as well.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2011年第5期418-421,共4页
Orthopedic Journal of China
关键词
转化生长因子
椎间失稳
软骨终板
退变
transforming growth factors
intervertebral destabilization
cartilage endplate
degeneration
