摘要
目的探讨金属硫蛋白(MT)2A基因多态性与饮水型地方性砷中毒易感性的关系。方法于2007年选择新疆奎屯饮水型地方性砷中毒病区饮用高砷水5年以上的慢性砷中毒患者共96人作为病例组;选择该病区饮用砷含量正常水源5年以上的健康人群共73人作为内对照组;选择非病区正常健康人群共89人作为外对照组。应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术进行MT2A基因rs10636位点(CC、CG、GG基因型和C、G等位基因)多态性的检测。采用Logisitic回归分析不同基因型与饮水型地方性砷中毒发病风险的关系。结果 MT2A基因rs10636位点CC、CG、GG各基因型频率在三组间的分布差异有统计学意义(χ2=16.353,P<0.05);而C、G等位基因频率在三组间的分布,差异无统计学意义。经Logistic回归分析,在调整年龄、性别、饮水年限后,女性携带CG+GG(杂合型+突变纯合型)基因型的概率体是男性的1.941倍(95%CI=1.086~3.471)。结论 MT2A基因rs10636多态位点的基因型和饮水型地方性砷中毒有关,性别是影响MT2A基因rs10636位点多态性的危险因素。MT2A基因rs10636位点可以作为饮水型地方性砷中毒的生物标志。
Objective To investigate the relationship between genetic polymorphism in MT2A rs10636 locus in chromosome 16 and susceptibility of water-born endemic arsenism. Methods MT2A gene polymorphism were analyzed by polymerase chain reaction restriction fragment length polymorphism technique(PCR-RFLP) among 96 patients with arsenism (case group), 73 healthy individuals (intra-control,IC) and 89 unrelated healthy individuals (extra-control, EC) in 2007. Results The distributions of genotype frequencies of rs10636 were deviated from the Hardy-Weinberg equilibrium (P〈0.05); The difference of MT2A rs10636 CG,GG and CG genotype in three groups was statistically significant (χ2=16.353,P〈0.05). Binary logistic regression analysis showed: gender could effect MT2A genetic polymorphism (P〈0.05),the mutation superiority of female was higher than male by 1.941 times. Conclusions MT2A (rs10636) gene may be associated with water-born endemic arsenism,and can be used as a biomarker in the related study.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2011年第2期99-102,共4页
Journal of Environment and Health
基金
国家自然科学基金(30960323)