摘要
物种的DNA C-值具有重要的生态意义,同时也具有种的特征,目前DNA C-值越来越广泛地应用于物种倍性和杂种鉴定、植物生态适应能力分析等。本文应用流式细胞术,以油菜(Brassica campes-tris)为材料,从标样、细胞核悬液、样品生长时期等方面研究了影响DNA C-值检测的实验因素,发现标样种类和样品的生长时期对实验结果没有明显影响;与Galbraith缓冲液和Otto缓冲液相比,应用Tris-MgCl2细胞核悬液能够得到稳定的实验结果。
DNA C-value is of important ecological significance and no much variation for species.The value has been widely applied in the study in relation to species polidy and hybrid identification,and ecological adaptability,etc.The present paper brief summarized the main methods to determine plant DNA-C value,proposed a modified method to determine the DNA C-value of plant by using the method of flow cytometry,and taking Brassica campestris as experimental material.The factors influencing the determination of DNA C-value were also analyzed from internal standard,nuclei suspension,and samples growth period.We found that species and growth period of the samples have no significant effect on the experimental results,Application of Tris-MgCl2 nuclei suspension liquid could produced stable experimental results compared with other two buffers.
出处
《植物检疫》
北大核心
2011年第2期40-44,共5页
Plant Quarantine
基金
质检公益项目(200810787)
国家自然科学基金项目(31070479)
上海市科委基础研究重点项目(10JC1412100)
上海市教委科技创新重点项目(06ZZ20
10ZZ81)
上海市教育委员会重点学科建设项目(J50401)
关键词
油菜
流式细胞术
核悬液
标样
稳定性
Brassica campestris
flow cytometry
nuclear suspension
internal standard
stability
