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分泌蛋白在痰液及培养液中鉴定结核分枝杆菌复合群的应用研究 被引量:9

STUDY ON THE APPLICATION OF SECRETOR GLOBULIN FOR IDENTIFYING MYCOBACTERIA TUBERCULOSIS COMPLEX IN SPUTUM AND CULTURE
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摘要 目的评价两种结核菌分泌蛋白试剂分别应用于痰液和培养液中鉴定结核分枝杆菌复合群(MTC)的效果。方法①采用结核分枝杆菌多克隆分泌蛋白试剂对20例健康人呼吸道分泌物以及151例疑为结核病病人痰标本进行检测,同时使用MGIT 960培养仪分离培养,阳性分离菌株进行传统方法鉴定,并使用结核分枝杆菌单克隆分泌蛋白MPT64鉴定。②23株分枝杆菌标准菌株采用MPT64单克隆试剂鉴定。③找出两种试剂的检出限。稀释H37RV标准菌株找出MPT64单克隆试剂的检出限,分析涂片阳性级别与多克隆分泌蛋白试剂结果关系找出该检出限并作比对。结果 MPT64单克隆试剂与多克隆分泌蛋白试剂的灵敏度、特异性、阳性预测值、阴性预测值分别为98.7%、100.0%、88.9%、98.7%和81.0%、85.9%、83.1%、84.0%、83.6%。MPT64单克隆试剂在3 000 CFU/ml的菌液浓度检出。多克隆分泌蛋白试剂在5 000~10 000 CFU/ml的阳性痰中检出。两者的平均检测时间为12 d和1 d。结论应用MPT64单克隆分泌蛋白试剂检测鉴定培养液中MTC是一种准确、快速、简便而又不需要任何仪器设备的实用型方法,值得临床推广应用;而多克隆分泌蛋白试剂可直接应用于痰液中检测鉴定MTC,尽管在灵敏度、特异性、准确度上未尽人意,但其快速性仍不失为一种让人期待的方法。
机构地区 广州市胸科医院
出处 《现代医院》 2011年第2期17-19,共3页 Modern Hospitals
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