摘要
对玻璃化冻存后羊Ovis aries软骨细胞的存活率和琥珀酸脱氢酶（SDH）活性进行了初步研究。结果表明：细胞存活率随着冻存时间的延长而逐渐下降,但下降速度不明显;SDH活性在玻璃化冻存1~20 d变化幅度较大,20~30 d下降趋势减弱,保持相对稳定。从各项指标来看,认为玻璃化溶液VSb组的冻存效果最好,在经过5,10,15,20和30 d的冻存后,其存活率分别为85.47%±1.78%,80.73%±1.81%,78.62%±2.06%,76.35%±2.58%和73.83%±1.49%,细胞SDH活性吸光度值分别为0.49±0.064,0.444±0.073,0.394±0.039,0.354±0.082和0.339±0.053。该玻璃化冻存方法对长期冻存羊软骨细胞具有一定的实际应用意义。
The effects of vitrification on survival rate and succinate dehydrogenase（SDH） activity of ovine chondrocytes were studied.Results showed that the SDH activity decreased remarkably within 20 d after vitrification,whereas the survival rate decreased slowly.VSb selected according to the trend of survival rates and vitality of SDH.After being cryopreserved for 5,10,15,20 and 30 days,the survival rates were 85.47% ± 1.78%,80.73% ± 1.81%,78.62% ± 2.06%,76.35% ± 2.58% and 73.83% ± 1.49%,and the vitality of SDH showed 0.49 ± 0.064,0.444 ± 0.073,0.394 ± 0.039,0.354 ± 0.082 and 0.339 ± 0.053.This indicated that cryopreservation of ovine chondrocytes by vitrification had some practical value.
出处
《浙江农林大学学报》
CAS
CSCD
北大核心
2011年第2期269-274,共6页
Journal of Zhejiang A&F University
基金
国家自然科学基金资助项目(50606032)
浙江省自然科学基金资助项目(Y304053)
关键词
动物学
玻璃化冻存
软骨细胞
存活率
琥珀酸脱氢酶
绵羊
zoology
vitrification
chondrocytes
survival rate
succinate dehydrogenase（SDH）
Ovis aries
