摘要
LCDV-cn是分离自中国养殖牙鲆的淋巴囊肿病病原,给中国牙鲆养殖业带来了严重危害。通过PCR、克隆和测序,获得了LCDV-cn部分基因的序列,证明LCDV-cn与LCDV-C的基因组序列是一致的。比较基因组学分析发现,LCDV-cn基因组中有137个ORF是其独有的、与LCDV-1及其它虹彩病毒无同源区域。对LCDV-cn基因组进行生物信息学分析发现,在LCDV-cn独有的ORF中,有的编码与宿主同源的蛋白,有的编码与DNA复制相关的蛋白,某些ORF编码的蛋白含有跨膜区等。LCDV-cn的独有基因或许对其毒力和宿主范围有关。
Lymphocystis disease virus-Chinese isolate（LCDV-cn） is the causatice agent of lymphocystis disease in paralichthys olivaceus,causing a huge loss in flounder aquaculture.Some gene sequences of LCDV-cn has been acquired by PCR,cloning and sequencing.Those sequences were consistent with LCDV-C.Found through comparative analysis,137 ORF is LCDV-cn’s own unique genes without homology with LCDV-1 and other iridovirus.LCDV-cn genome was analyzed by bioinformatics methods.In proteins encoded by LCDV-cn’s unique genes,some protein was homologous with fish protein;some protein was related to DNA duplication;and some protein contained transmenmbrane domain,etc.It is likely that LCDV-cn’s unique genes influence its virulence and host range.This study can form the foundation for in-depth researches,such as mechanism of LCDV-cn’s pathopoiesia and infection,prevention and treatment of lymphocystis disease,and so on.
出处
《广东海洋大学学报》
CAS
2011年第1期1-6,共6页
Journal of Guangdong Ocean University
基金
国家高技术研究发展计划项目(2006AA100309)资助
