摘要
目的研究不同剂量雷帕霉素对小鼠体内Treg细胞的影响。方法将SPF级昆明系小鼠60只随机分为对照组(A)和实验组(B、C、D),B、C、D三组分别灌胃雷帕霉素1、2、3 mg.kg-1,A组每天予以无菌水灌胃,共3周。3周后,无菌条件下心脏采血,EDTA抗凝,分离脾脏,制备单细胞悬液,采用流式细胞仪检测小鼠外周血和脾脏中CD4+CD25+调节性T细胞水平(CD4+CD25+Treg细胞占CD4+T细胞的百分比)。结果实验组(B、C、D)小鼠外周血和脾细胞中CD4+CD25+Treg细胞水平分别为(9.62±1.43)%(、13.76±1.97)%(、15.41±2.45)%和(12.23±4.56)%(、23.03±6.18)%(、25.17±6.42)%,对照组(A)小鼠外周血和脾细胞中CD4+CD25+Treg细胞水平分别为(3.52±0.65)%和(6.53±3.01)%,无论是在外周血还是脾细胞中,B、C、D组CD4+CD25+Treg细胞水平明显高于A组(P<0.05),C、D组与B组之间CD4+CD25+Treg细胞水平也有明显差异性(P<0.05),C组和D组之间CD4+CD25+Treg细胞水平无明显差异性(P>0.05)。结论雷帕霉素能够诱导昆明系小鼠体内CD4+CD25+Treg细胞增殖,其使用剂量可以影响CD4+CD25+Treg细胞的增殖程度。
Aim To investigate how rapamycin（RAPA） at different doses levels induce Kunming-strain mouse CD4^＋CD25^＋Treg cells proliferations.Methods 60 Kunming-strain mice at the age of 8 weeks were divided into a control group（A） and three experimental groups（B,C,D）.The mice in groups B,C and D were fed RAPA 1,2 and 3 mg·kg-1 intragastric administration.The mice in group A were given sterile water as the control group.After three weeks,under sterile conditions by collecting the peripheral blood and then seperating the splenocytes（EDTA anticoagulant）,we were able to generate a single-cell suspension.The level of CD4^＋CD25^＋Treg cells in the mouse peripheral blood and splenocytes were detected by flow cytometer.（The ratio of CD4^＋CD25^＋Treg cells to CD4^＋CD25-Treg cells）.Results The CD4^＋CD25^＋Treg cells in the mouse peripheral blood and splenocytes of the experimental groups（B,C,D） were（9.62±1.43）%,（13.76±1.97）%,（15.41±2.45）% and（12.23±4.56）%,（23.03±6.18）%,（25.17±6.42）%.The CD4^＋CD25^＋Treg cells in the mouse peripheral blood and splenocytes of the control group（A） were（3.52±0.65）% and（6.53±3.01）%.The levels of the CD4^＋ CD25^＋ Treg cells either in the peripheral blood or splenocytes of the groups B,C and D were significantly higher than those in group A（P0.05）.There were significant differences among group C,D and B according to the level of the CD4^＋CD25^＋Treg cells（P0.05） while there were no significant differences between group C and D according to the level of the CD4^＋CD25^＋Treg cells（P0.05）.Conclusion RAPA can induce Kunming-strain mouse CD4^＋CD25^＋Treg cells proliferations in vivo and the level of CD4^＋CD25^＋Treg cells proliferations in vivo is influenced by the dose of RAPA used.
出处
《安徽医药》
CAS
2011年第3期284-286,共3页
Anhui Medical and Pharmaceutical Journal
