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用三维荧光光谱法测定8种植物油脂的荧光信息 被引量:14

FLUORESCENCE INFORMATION OF 8 KINDS OF VEGETABLE OILS BY 3D FLUORESCENCE SPECTROSCOPY
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摘要 选取花生油、大豆油、菜籽油、芝麻油、玉米油、葵花籽油、米糠油和棉籽油8种植物油脂共98个样品,采用Gary Eclipse荧光光度计,在激发狭缝和发射狭缝宽度为5 nm、激发波长在250~700 nm范围内以10 nm为间隔、发射波长在240~760 nm范围内的条件下扫描样品的发射光谱,获得每个样品的三维荧光光谱.用Origin7.5软件绘制光谱数据的二维等高线图,用于研究各种油脂的荧光信息.结果显示,8种油脂的荧光信息有显著差别. The paper determined the emission spectra and the 3D fluorescent spectra of 8 kinds of vegetable oils(98 samples),including peanut oil,soybean oil,rapeseed oil,sesame oil,corn oil,sunflower seed oil,rice bran oil and cottonseed oil,by using a Gary Eclipse fluorometer under the conditions of emission wavelength 240 nm to 760 nm,excitation wavelength 250 nm to 700 nm at an interval of 10 nm,excitation slit width 5 nm,and emission slit width 5 nm.We plotted the 2D contour map of each vegetable oil by Origin7.5 based on the fluorescent spectra data to study the fluorescence information of each vegetable oil.The results showed that the 8 kinds of vegetable oils had different fluorescence information.
出处 《河南工业大学学报(自然科学版)》 CAS 北大核心 2010年第6期36-39,共4页 Journal of Henan University of Technology:Natural Science Edition
基金 河南工业大学校科研基金重点项目(09XZD005)
关键词 植物油脂 三维荧光光谱 等高线光谱图 vegetable oil 3D fluorescence spectrum contour spectra map
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参考文献6

  • 1许金钩,王尊本.荧光分析法[M],北京:科学出版社,2006
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  • 3Konstantina I Poulli, George A Mousdis, Constantinos A Georgiou. Classification of ed- ible and lampante virgin olive oil based on synchronous fluorescence and total lumines- cence spectroscopy [ J ]. Analytica Chimica Acta, 2005, 542 : 151-156.
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