摘要
【目的】筛选适宜沙枣多酚纯化的大孔树脂。【方法】通过静态吸附与解析试验,从AB-8、NKA-9、NKA、D4020、X-5和D101中筛选可用于沙枣多酚纯化的大孔树脂,并研究其对沙枣多酚的动态吸附和解析性能。【结果】NKA-9树脂对沙枣多酚的饱和吸附量为19.58mg/g,吸附等温线符合Langmuirh和Freundlich方程,动力学曲线符合Langmuir方程,饱和吸附时间为7h左右;NKA-9大孔树脂纯化沙枣多酚的最佳工艺条件为:上柱液pH4,上柱液中沙枣多酚质量浓度1.00mg/mL,上样液体积25.12mL,上样流速1.0mL/min,洗脱液乙醇体积分数60%,洗脱剂体积18.84mL,洗脱剂流速1.0mL/min。【结论】NKA-9型大孔树脂表现出较好的吸附性能与解析效果,能很好地富集纯化沙枣多酚。
[Objective] This study was to study a suitable resin for purifying Elaeagnus angustifolia L.polyphenol.[Method] Dynamic absorption and desorption experiments were carried out to select suitable resin from AB-8,NKA-9,NKA,D4020,X-5 and D101 of macroporous absorbent resin for E.angustifolia L.polyphenol.[Result] The static saturated adsorption capacity of NKA-9 resin was 19.58 mg/g while the time for it to achieve saturation adsorption was 7 h.Besides,the absorption isotherm data of NKA-9 resin could well fit the Langmuir equation and Freundlich equation,and the absorption kinetic also fitted the Langmuir equation;The optimum absorption conditions of macroporous resin NKA-9 were pH value 4,concentration of feed 1.00 mg/mL,volume of polyphenol sample 25.12 mL,feeding rate 1.0 mL/min.And the the optimum desorption conditions were elute ethanol concentration 60%,elute volume 18.84 mL,elution rate 1.0 mL/min.[Conclusion] Macroporous resin NKA-9 possessed the strongest absorbend ability and easier desorption property,which is a better macroporous absorbent resin for purificating polyphenols in E.angustifolia L..
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2010年第12期215-220,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
兰州理工大学优秀青年教师培养计划资助项目(0908ZXC164)
关键词
沙枣
多酚
大孔吸附树脂
纯化
Elaeagnus angustifolia L.
polyphenol
macroporous absorbent resin
purification
