摘要
目的:测定野生与栽培川西獐牙菜中3种有效药用成分,即龙胆苦苷、芒果甙和齐墩果酸的含量.方法:采用高效液相色谱法分离和测定川西獐牙菜中龙胆苦苷、芒果甙和齐墩果酸的含量.流动相:甲醇-水-0.5%乙酸(25∶75∶0.8)、甲醇-0.5%乙酸(25∶75)和甲醇-水(90∶10);检测波长:270nm、254nm和215nm;流速:1.0mL/min;进样量:10μL.结果:栽培川西獐牙菜中龙胆苦苷、芒果甙和齐墩果酸含量与野生种基本相同.结论:栽培川西獐牙菜完全可以代替野生种.
Aim： Three kinds of effectively medical component such as oleaolic acid,mangiferin and gentiopicroside of artificial and wild Swertia mussotii Franch were determined.Method： The content of oleaolic acid,mangiferin and gentiopicroside of Swertia mussotii Franch were separated and determined by HPLC.The conditions of HPLC were as follows： mobile phase,methanol-water-0.5（25：75：0.8）,methanol-0.5（25：75） and methanol-water（90∶10）;detectived wave length,270nm,254nm and 215nm;flowing rate,1.0mL/min;injection volume,10μL.Result： The content of oleaolic acid,mangiferin and gentiopicroside of wild and artificial Swertia mussotii Franch was the same.Conclusion： The wild Swertia mussotii Franch was completely replaced by the artificial one.
出处
《青海师范大学学报（自然科学版）》
2010年第4期57-60,共4页
Journal of Qinghai Normal University(Natural Science Edition)
关键词
HPLC
川西獐牙菜
龙胆苦苷
芒果甙
齐墩果酸
high performance liquid chromatography
Swertia mussotii franch
gentiopicroside
mangiferin
oleaolic acid
