摘要
目的通过对入境集装箱中发现的死鼠进行实验室检测,加强口岸对入境啮齿动物携带鼠疫杆菌的监测力度。方法分别用胶体金和PCR方法对入境集装箱中发现的死鼠进行鼠疫杆菌检测。结果该样本鼠疫杆菌F1抗原阳性,pla基因和fra基因PCR扩增均为阳性,判定该样本为疑似鼠疫杆菌感染。结论在口岸一线加强对入境鼠类携带鼠疫杆菌检测具有重要意义。同时,应不断加强口岸公共卫生核心能力建设,加强公共卫生实验室建设及重大疫情检测技术储备。
Objective To conduct the laboratory diognoses of dead mouse found in entry container,and to strengthen the monitoring efforts to entry rodent carring plague bacteria.Methods The gold immunochromatographic assay（GICA） and PCR methods were used to test a dead mouse about Yersinia pestis which was found in import container at Daxie port.Results Yersinia pestis F1 antigen,pla gene and fra gene amplified by PCR method were positive so the sample may be infected by Yersinia pestis.Conclusions It has great significance in strengthening the detection of Yersinia pestis to entry mouse at port.
出处
《中国国境卫生检疫杂志》
CAS
2011年第1期17-18,62,共3页
Chinese Journal of Frontier Health and Quarantine
