摘要
【目的】观察耳穴疗法(耳甲区)对糖尿病大鼠心肌细胞超微结构的影响。【方法】选用SPF级SD大鼠50只,设正常组10只,另40只大鼠采用腹腔注射链脲佐菌素(STZ,剂量为55 mg/kg)法复制糖尿病大鼠模型,造模成功后随机分4组:耳穴组选用皮内针埋入耳甲区治疗,埋针时间为30 min,每天治疗1次;二甲双胍组以盐酸二甲双胍(剂量为52 mg.kg-1.d-1)分2次灌胃;二甲双胍加耳穴组采用以上2种治疗方法同时使用,治疗时间均为30 d;模型组大鼠未进行任何治疗。5组大鼠均采集空腹血检测血糖,30 d后复查血糖,并采用透射电镜观察各组大鼠的心肌细胞超微结构。【结果】各组大鼠治疗前空腹血糖值与正常组比较差异均有显著性意义(P<0.01);正常组和模型组治疗后血糖值与治疗前比较差异均无显著性意义(P>0.05);二甲双胍组、耳穴组及二甲双胍加耳穴组治疗后空腹血糖值显著下降,与模型组及治疗前比较差异均有显著性意义(P<0.05)。电镜检查结果显示二甲双胍加耳穴组拮抗心肌细胞病变的效果最佳,单纯使用二甲双胍也有较好的疗效,耳穴组与模型组比较心肌病变有一定程度的改善,但作用不及前2组。【结论】耳穴治疗能有效降低血糖,与二甲双胍合用对糖尿病心肌病理变化具有一定的保护作用。
Objective To investigate the effect of ear-point needle-embedding therapy myocardial cells in streptozotocin-induced diabetic rats. Methods Ten Sprague-Dawley male on ultrastructure of rats were in normal group. Fourty Sprague-Dawley male rats with specific pathogen free received intraperitoneal injection of streptozotocin, and then were equally randomized into four groups： ear-point treatment group （receiving ear-point needle-embedding therapy in auricular concha area for 30 min once per day） , metformin treatment group （ gastric gavage of metformin 52 mg·kg^-1·d^-l·bid）, combined treatment group （receiving both ear-point needleembedding treatment and metformin） and model group. The treatment lasted 30 days. Before and after treatment, fasting blood glucose （FBG） of all rats was measured. The uhrastructure of myocardial ceils was examined under transmission electron microscope. Results Before treatment, FBG in model rats was higher than that in the normal group （P 〈 0.01 ）. Before and after treatment, the difference of FBG was insignificant in the model group and the normal group （ P 〉 0. 05 ）. FBG in ear-point treatment group, metformin treatment group and combined treatment group were decreased after treatment （P 〈 0. 05 compared with that before treatment and with that in the model group after treatment）. The results under transmission electron microscope showed that the improvement of pathological changes of myocardial cells was the best in the combined treatment group, better in mefformin treatment group, and good in ear-point treatment group. Conclusion Ear-point needle-embedding therapy has an effect on decreasing blood glucose, and ear-point needle-embedding treatment combined with metformin has protective effect on myocardial cells in streptozotocin-induced diabetic rats.
出处
《广州中医药大学学报》
CAS
2011年第2期145-148,共4页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(编号:30873300)
