摘要
目的探讨十全大补汤总多糖(以下简称总多糖)的体外抗肿瘤作用,为其临床应用及抗肿瘤药物的研发提供依据。方法以200、100、50μg/ml浓度总多糖干预体外培养的人肺癌A549细胞株、人肝癌Bel-7402细胞株、人结肠癌HCT-8细胞株和人宫颈癌Hela细胞株。作用24、48、72 h时采用MTT法测定细胞增殖抑制率(IR)。结果 200μg/ml总多糖作用48 h时A549细胞的IR最高;总多糖对另3种细胞的作用均为随浓度及作用时间延长IR逐渐升高,以200μg/ml、作用72 h时IR最高,呈剂量及时间依赖性。结论总多糖可在体外抑制肿瘤细胞生长;其有望作为抗肿瘤药物用于临床。
ObjectiveTo investigate the anti-tumor effects of total polysaccharide（TPS） extracted from Shiquan Dabu Decoction in vitro,so as to provide basis for its clinical application and research of anti-tumor drugs.MethodsThe human lung cancer cells A549,hepatoma cells Bel-7402,colorectal carcinoma cells HCT-8 and cervical carcinoma cells Hela cultured in vitro were treated with TPS of 200,100,50 μg/ml,the inhibitory rate of proliferation were measured by MTT assay at 24,48,72 h after the treatment.ResultsIR reached the highest in the A549 cells when treated with TPS of 200 μg/ml for 48 hs;IR in the other three kinds of cells increased gradually with the increasing of TPS concentration and prolonging of treating time,reached the highest at 200 μg/ml for 72 hs,which showed dose and time-dependent manner.ConclusionsTPS could inhibit the growth of tumor in vitro;and hope to be used for the treatment of tumor as anti-tumor drugs.
出处
《山东医药》
CAS
北大核心
2011年第7期15-17,共3页
Shandong Medical Journal
基金
河北大学医学部科学研究基金项目(2007Y06)
关键词
十全大补汤
总多糖
肿瘤细胞
增殖抑制
total polysaccharide extracted from Shiquan Dabu Decoction
spleen cell
inhibition of proliferation
