摘要
目的探讨血小板活化因子(PAF)与实验大鼠肝脏病变的关系及银杏制剂的治疗作用。方法将实验用Wistar大鼠60只,随机分成正常对照组(12只)、肝纤维化模型组(16只)、银杏制剂低剂量治疗组(16只)及银杏制剂高剂量治疗组(16只)4组。除正常对照组外,余3组应用CCl4皮下注射造模,2次/w,共12 w。正常对照组及模型组每日用生理盐水灌胃,而治疗组则每日用银杏制剂灌胃。12 w后,将全部大鼠处死,留取血清、肝组织,测定肝功能(ALT、ALB)、血清PAF及各种肝纤维化指标(HA、PⅢP、C-Ⅳ、LN),同时行组织病理学检查,并行胶原纤维增生程度半定量分析。结果模型组与正常对照组比较,除白蛋白降低外,血清PAF、ALT及各种肝纤维化指标均升高;各治疗组与模型组间比较,除白蛋白升高外,余各项指标均下降,同时肝脏炎症及肝纤维化程度减轻,上述指标差异均有统计学意义。结论 PAF与肝脏损伤及肝脏纤维化的发生有明显关系,PAF拮抗剂——银杏制剂有明显抑制和逆转肝纤维化的作用。
Objective To investigate the relationship of platelet activating factor（PAF）and liver damage in rats and the therapeutic effects of ginkgo preparation.Methods 60 Wistar rats were randomly divided into four groups：normal control group（n=12）,liver fibrosis model group（model group,n=16）,low dose gingko-treated group（n=16）,high dose gingko-treated group（n=16）.Except the normal control group,rats in other three groups were hypodermically injected with carbon tetrachloride（CCl4）twice a week.12 w later,the pathological examination showed that the model of hepatic fibrosis was successfully established.After that,the normal control group and the model group were intragastrically administered with normal saline every day and the two treated group were perfused with Ginkgo preparation at 50 or 100 mg/（kg·d）,respectively.12 w later,all the rats were sacrificed and their liver tissues and sera were collected.Alanine aminotransferase（ALT）and albumin（ALB）,serum PAF,hyaluronic acid（HA）,pro-collagen peptide type Ⅲ（PⅢP）,collagen type Ⅳ（C-Ⅳ）and laminin（LN）were measured.Meanwhile,pathological examination was performed for the semiquantitative analysis of the degree of liver fibrosis.Results Compared with the normal control group,PAF,ALT,PⅢP,C-Ⅳ,LN and HA increased significantly in the model group except that ALB reduced.Compared with the model group,all indexes in the two treated groups decreased significantly except that ALB increased,and the degree of liver inflammation and fibrosis mitigated significantly.These differences all had statistic significance.Conclusion PAF appears to be apparently associated with liver damage and liver fibrosis.Ginkgo preparation,a PAF antagonist,can obviously inhibit and reverse liver fibrosis.
出处
《西南国防医药》
CAS
2011年第3期246-249,共4页
Medical Journal of National Defending Forces in Southwest China
