摘要
经过反复试验,摸索出一种提取高质量植物基因组DNA的方法:改良的4×CTAB法。以盐肤木叶片为实验材料,提取到高质量的基因组DNA,建立了酶切、连接、预扩增、选择性扩增的AFLP反应体系。通过两种引物组合"E+3/M+3"和"E+2/M+3"策略筛选出8对条带分辨率高、多态性好的引物组合,优化了盐肤木的AFLP银染反应体系,得到了清晰的AFLP指纹图谱,为盐肤木种群遗传多样性研究奠定了基础。
A modified method（4×CTAB）was used to extract the effective genomic DNA from the leaves of Rhus chinensis,and the systems of DNA restriction reaction,ligase reaction,pre-amplification and selective amplification reaction were established in this study.Using the two kinds of primer-combined methods（E＋3/M＋3 and E＋2/M＋3）,8 pairs of primers were finally selected to amplify the genomic DNA of R.chinensis,and clear bands with high genetic polymorphism and difference were obtained.The present results provided a strong foundation for the further examination of population genetic diversity and structure of R.chinensis using AFLP method.
出处
《广西植物》
CAS
CSCD
北大核心
2011年第1期36-38,42,共4页
Guihaia
基金
国家自然科学基金(30670361)
山西省自然科学基金(2007011078)~~
